NHDF Epi5 Cl5 iPSC

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name BIHi004-A
Alternative name(s)
NHDF Epi5 Cl5 iPSC
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 10th December 2018
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Provider

Generator Berlin Institute of Health (BIH)
Owner Berlin Institute of Health (BIH)
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA103992834
Cellosaurus CVCL_IT55

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research
Subclones

Donor Information#

General Donor Information

Sex female
Ethnicity hispanic

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA103992835

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name CC-2511
Derived from same source line (potentially other lot and donor, see below):
Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Source cell type (free text) NHDF-Ad-Der Fibroblasts
Source cell line lot number
0000421626
Age of donor (at collection) 35-39
Collected in 2014
Source cell line vendor LONZA

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Unkown

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Morphology pictures
Differentiation Potency
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,add(12)(q22)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)