Generation of an iPSC-line (BIONi010C-48) with restored P-glycoprotein functionality following transfection with the human MDR1 gene in the AAVS1 locus
Summary
The human MDR1 gene encodes the efflux transporter P-glycoprotein, which plays an important part of the blood-brain barrier function of brain microvascular endothelial cells (BMECs). Here, we report the generation of an iPSC line, where a construct of the human MDR1 gene was inserted into the safe-site locus AAVS1. This iPSC line (BIONi010-C-48) shows functional expression of P-gp and can further be differentiated and cultured into electrically tight BMEC-like monolayers exhibiting polarized expression of P-gp in the apical membrane. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Nielsen AKR, Lilieholm-Røngren L, Schmid B, Holst B, Brodin B, Saaby L |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103348 |
| PubMed | 38364505 |
| DOI | 10.1016/j.scr.2024.103348 |