408737 CB9

General

Cell Line

hPSCreg name UMi042-A-1
Cite as:
UMi042-A-1 (RRID:CVCL_D0NU)
Alternative name(s)
408737 CB9
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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APOE, APOE
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Last update 22nd May 2023
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Provider

Generator University of Miami - Miller School of Medicine (UM)
Owner University of Miami - Miller School of Medicine (UM)
Derivation country United States

External Databases

BioSamples SAMEA112485561
Cellosaurus CVCL_D0NU
Wikidata Q123033745

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity African American

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Stage
clinically diagnosed with Alzheimer's disease
Synonyms
  • Alzheimer's Disease
  • Alzheimer disease
  • Alzheimer Disease
  • Alzheimer dementia
  • Alzheimer's Dementia
  • Alzheimer's disease
  • Alzheimer's disease, unspecified
show more synonyms
Family history unknown

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA112484915

Ethics

Also have a look at the ethics information for the parental line UMi042-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line UMi042-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFLEX
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
OCT4
Yes
SOX2
Yes
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Alzheimer's Disease
  • Alzheimer disease
  • Alzheimer Disease
  • Alzheimer dementia
  • Alzheimer's Dementia
  • Alzheimer's disease
  • Alzheimer's disease, unspecified
show more synonyms
Genetic modifications
APOE (target)
Isogenic modification
19q13.2
NM_001302688.2:c.466T>C
NP_001289617.1:p.Cys156Arg
Homozygous
The ApoE4/4 alleles have been edited in to a APOE3/3 alleles
Repaired
APOE4-3-UMI042-A-1.pdf
Sanger Sequencing
Genetic modifications not related to a disease
Variant
19q13.2
NM_001302688.2:c.474C>G
NP_001289617.1:p.Arg158Arg
Homozygous
A synonymous mutation was placed at the PAM site