408737 CB9
UMi042-A-1
General
Cell Line |
|
| hPSCreg name | UMi042-A-1 |
| Cite as: | UMi042-A-1 (RRID:CVCL_D0NU) |
| Alternative name(s) |
408737 CB9
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
BIONi037-A-2 (BIONi037-A ApoE2/2 #M10-7) BIONi037-A-3 (BIONi037-A ApoE3/4 #P10-22) BIONi037-A-4 (BIONi037-A ApoE4/4 #I10-53) BIONi037-A-1 (16423 ApoE KO) BIONi010-C-2 (BIONi010-C ApoE E3/E3 #H8 P32) BIONi010-C-3 (BIONi010-C ApoE KO #KO30 P30) BIONi010-C-4 (BIONi010-C ApoE E4/E4 #B44 P27) BIONi010-C-6 (BIONi010-C ApoE E2/E2) |
| Last update | 22nd May 2023 |
| User feedback | |
Provider |
|
| Generator | University of Miami - Miller School of Medicine (UM) |
| Owner | University of Miami - Miller School of Medicine (UM) |
| Derivation country | United States |
External Databases |
|
| BioSamples | SAMEA112485561 |
| Cellosaurus | CVCL_D0NU |
| Wikidata | Q123033745 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | African American |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
| Family history | unknown |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
|
External Databases (Donor) |
|
| BioSamples | SAMEA112484915 |
Ethics
Also have a look at the ethics information for the parental line
UMi042-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
|
The source cell information can be found in the parental cell line
UMi042-A.
|
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFLEX
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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