General

Cell Line

hPSCreg Name
UMi038-A-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th May 2022
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Provider

Generator University of Miami - Miller School of Medicine (UM)
Owner University of Miami - Miller School of Medicine (UM)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA14341487
CLO CLO_0103884

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity non-Hispanic White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alzheimer disease (primary disease)
Alzheimer Disease
Family history Yes
Is the medical history available upon request? no
Is clinical information available? yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA14096005

Ethics

Also have a look at the ethics information for the parental line UMi038-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? ThermoFisher SCientific
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line UMi038-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Accutase
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFLEX(TM)
Main protein source:
Serum concentration: 0 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Alzheimer disease
Genetic modifications
SORL1 sortilin related receptor 1 [ Homo sapiens (human) (target)
Isogenic modification
Chr11:121591080
NM_003105.6:c.4293del
NP_003096.2:p.Cys1431fs
Heterozygous
The deletion in SORL1 (SORL1/Chr11:121591080 (hg38) dbSNP: rs1343336951; Heterozygous frameshifting deletion of cytosine at position 4293 of coding DNA, c.4293delC; p.Cys1431TrpfsX2) has been corrected into the normal sequence using CRISPR/CAs9 genome editing.
Repaired