UMi038-A-1
General
Cell Line |
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| hPSCreg name | UMi038-A-1 |
| Cite as: | UMi038-A-1 (RRID:CVCL_C0YZ) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 5th May 2022 |
| User feedback | |
Provider |
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| Generator | University of Miami - Miller School of Medicine (UM) |
| Owner | University of Miami - Miller School of Medicine (UM) |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA14341487 |
| Cellosaurus | CVCL_C0YZ |
| Wikidata | Q114313199 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | non-Hispanic White |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | Yes |
| Is the medical history available upon request? | no |
| Is clinical information available? | yes |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has sister:
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External Databases (Donor) |
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| BioSamples | SAMEA14096005 |
Ethics
Also have a look at the ethics information for the parental line
UMi038-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | ThermoFisher SCientific |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UMi038-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFLEX(TM)
Main protein source: Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| SOX17 SRY-box transcription factor 17 |
Yes |
| FOXA2 forkhead box A2 [ Homo sapiens (human) ] |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| SMA: ACTA2 actin alpha 2, smooth muscle [ Homo sapiens (human) ] |
Yes |
| TBXT T-box transcription factor T [ Homo sapiens (human) ] |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| SOX1 SRY-box transcription factor 1 [ Homo sapiens (human) ] |
Yes |
| PAX6 paired box 6 [ Homo sapiens (human) ] |
Yes |
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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