CSBZZU_PS1_001
CSBZZUi001-A
General
Cell Line |
|
| hPSCreg name | CSBZZUi001-A |
| Cite as: | CSBZZUi001-A (RRID:CVCL_E3TU) |
| Alternative name(s) |
CSBZZU_PS1_001
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 27th March 2024 |
| Notes | Ipsc is derived from patients with early-onset AD who carry the PS1 gene mutation, F237L |
| User feedback | |
Provider |
|
| Generator | The first affiliated Hospital of Zhengzhou University (CSBZZU) |
| Owner | The first affiliated Hospital of Zhengzhou University (CSBZZU) |
| Distributors | |
| Derivation country | China |
External Databases |
|
| BioSamples | SAMEA115444276 |
| Cellosaurus | CVCL_E3TU |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Han |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA115444275 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | 13707656096 |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics Committee of the First Affiliated Hospital of Zhengzhou University |
| Approval number | 2023-KY-0090-003 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Passage method |
Enzymatically
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 95 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Marker | Present | Absent |
| mCpG | ||
| OCT4 | X |
Report
Morphology pictures
Self-renewal
Unknown
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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