Generation of an induced pluripotent stem cell line (CSBZZUi001-A) from a female Alzheimer's patient carrying the PSEN1 709 T > C heterozygous mutation
Summary
Pluripotent stem cells were generated through the electroporation of episomal plasmids, containing crucial reprogramming factors, into skin fibroblasts extracted from a female Alzheimer's patient harboring the PSEN1 709 T > C (p.Phe237Leu) heterozygous mutation. The pluripotent stem cells exhibit a normal karyotype and express pivotal stem cell markers including TRA-1-60, Nanog, SOX2, and OCT4. Furthermore, their capacity to differentiate into the three germ layers in in vivo teratoma experiments has been substantiated. The pluripotent stem cell line can serve as a cellular model for Alzheimer's disease, offering significant value in elucidating the pathogenesis and therapeutic strategies of the disease. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Meng XY, Sun HF, Zhang YJ, Li WX, Wang DD, Liu DH, Li SM, Wu Y, Li JK, Liu XH, Liu PP, Yang RZ, Li SA, Kang JS |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Sep;79:103486 |
| PubMed | 38968760 |
| DOI | 10.1016/j.scr.2024.103486 |