SIAISi008-A

ZMY

General

Cell Line

hPSCreg Name
SIAISi008-A
Alternative name(s)
ZMY
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 23rd November 2020
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Provider

Generator Shanghai Institute for Advanced Immunochemical Studies (SIAIS) (SIAIS)
Owner Shanghai Institute for Advanced Immunochemical Studies (SIAIS) (SIAIS)

External Databases

BioSamples SAMEA6880594
Cellosaurus CVCL_ZA32
CLO CLO_0103096
Wikidata Q98132567

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Chinese Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
obsolete_Alzheimer's disease
The donor is a carrier of a disease-associated mutation and not affected.
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer Disease
  • Alzheimers Dementia
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA6880595

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: wangying1@shanghaitech.edu.cn
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink wangying1@shanghaitech.edu.cn
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Shanghai Ruijin Hospital Ethics Committee
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Shanghai Ruijin Hospital Ethics Committee
Approval number
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
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Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
OCT3/4
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
AFP
Unknown
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
SMA-1
Unknown
Morphology
Ectoderm
Ont Id: UBERON_0000924
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)