Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer's disease (AD) patient and her two unaffected children from Chinese Han population


A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The iPSC line displayed a normal karyotype. This model provides insight into further pathological studies to research identify early biomarkers, study disease pedigrees, and also for drug testing purposes. Copyright © 2021. Published by Elsevier B.V.

Authors Zhang W, Dai Q, Hua Y, Di W, Guo J, Zhao J, Deng Y, Wang Y
Journal Stem cell research
Publication Date 2021 Mar;51:102172
PubMed 33472125
DOI 10.1016/j.scr.2021.102172

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