SIAISi016-A

General

Cell Line

hPSCreg Name
SIAISi016-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th May 2022
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Provider

Generator Shanghai Institute for Advanced Immunochemical Studies (SIAIS) (SIAIS)
Owner Shanghai Institute for Advanced Immunochemical Studies (SIAIS) (SIAIS)

External Databases

Cellosaurus CVCL_B3S2
BioSamples SAMEA14366679
CLO CLO_0103864

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alzheimer's Disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Alzheimer's Disease
  • Alzheimer disease
  • Alzheimer Disease
  • Alzheimer dementia
  • Alzheimer's Dementia
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA14366682

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: 1253950497@qq.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ruijin Hospital Ethics Committee
Approval number KY2020-66
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral blood mononuclear cell
Synonyms
  • Peripheral blood monocyte
Source cell origin
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46.XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)