General

Cell Line
hPSCreg name	UMi050-B
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	UMi050-B
Alternative name(s)	417027 clone 3
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	7th May 2025
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Provider
Generator	University of Miami - Miller School of Medicine (UM)
Derivation country	United States
External Databases
BioSamples	SAMEA118259533
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed

Donor Information

General Donor Information
Sex	male
Ethnicity	Hispanic
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Alzheimer disease The donor is affected. Synonyms AD Alzheimer dementia Alzheimer disease Alzheimer's dementia Alzheimer's disease Alzheimers dementia Alzheimers disease presenile and senile dementia Alzheimer disease, familial show more synonyms show less synonyms
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	Yes
Donor Relations
Other cell lines of this donor	UMi050-A
External Databases (Donor)
BioSamples	SAMEA118259374

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	No
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Alternatives to consent are available?	No
Is there other documentation provided to the donor for consenting purposes?	No
Confirm that consent was obtained by a qualified professional	Yes
Has the donor agreed to be re-contacted?	Unknown
Has the donor been informed about how her/his data will be protected?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
* Does consent expressly prevent the derivation of pluripotent stem cells?	No
* Does consent pertain to a specific research project?	No
Does consent permit unforeseen future research, without further consent?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
How may genetic information associated with the cell line be accessed?	Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	University of Miami Internal Review Board
Approval number	MOD00012691
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Derived Peripheral Blood Mononuclear Cell Mononuclear cells collected from peripheral blood. Synonyms Peripheral Blood Mononuclear cells Derived Peripheral Blood Mononuclear Cell
Source cell origin	Blood A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood. Synonyms Reticuloendothelial System, Blood Peripheral Blood peripheral blood blood Blood Whole Blood BLOOD portion of blood vertebrate blood show more synonyms show less synonyms
Reprogramming method
Vector type	Non-integrating
Vector	Sendai virus
Is reprogramming vector detectable?	No
Methods used	RT-PCR
Files and images showing reprogramming vector expressed or silenced	2025-04-04_SeV_RT-PCR_Test_Results_LOY.pdf
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Vitronectin
Feeder cells	No
Passage method	Enzyme-free cell dissociation EDTA
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes	–
SOX2	Yes	–

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 45,X Passage number: 12 Karyotyping method: G-Banding
Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?	Yes