UMi039-A-1
General
Cell Line |
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| hPSCreg name | UMi039-A-1 |
| Cite as: | UMi039-A-1 (RRID:CVCL_C0Z0) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
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| Last update | 10th May 2023 |
| User feedback | |
Provider |
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| Generator | University of Miami - Miller School of Medicine (UM) |
| Owner | University of Miami - Miller School of Medicine (UM) |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA110371900 |
| Cellosaurus | CVCL_C0Z0 |
| Wikidata | Q114313202 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | non-Hispanic White |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Family history | Yes |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
46XX
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has sister:
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External Databases (Donor) |
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| BioSamples | SAMEA14096008 |
Ethics
Also have a look at the ethics information for the parental line
UMi039-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Thermo Fisher Scientific |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | THese lines can be used for academic research purposes only and not for commercial applications. |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UMi039-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | Embryonic stem cell like morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
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| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFlex(TM) media
Main protein source: Serum concentration: 0 % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 25
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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