15M0014

General

Cell Line

hPSCreg name TUSMi006-A
Cite as:
TUSMi006-A (RRID:CVCL_VN40)
Alternative name(s)
15M0014
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKBi011-A-1
(iLB-AD + ApoE KO)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UKBi011-A-2
(ApoE 2/2)
Donor's gene variants:
APOE, APOE
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Alzheimer disease
UKBi011-A-3
(ApoE 3/3)
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APOE, APOE
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Alzheimer disease
UKBi011-A-4
(ApoE 3/4)
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APOE, APOE
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Alzheimer disease
STBCi006-A-1
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APOE, APOE
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Alzheimer disease
STBCi006-A-3
(ApoE 3/3)
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APOE, APOE
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Alzheimer disease
STBCi006-A-4
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APOE, APOE
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Alzheimer disease
MUNIi013-A
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IPTi003-A
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BIOTi001-A
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obsolete_Alzheimer's disease
MUNIi011-A
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Alzheimer's Disease
MUNIi012-A
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obsolete_Alzheimer's disease
SIAISi006-A
(JJY)
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Alzheimer's Disease
UMi043-A
(AD 381852 clone #1)
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Alzheimer's Disease
STBCi071-C
(SFC057-07-02)
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Alzheimer disease
ZZUi013-A
(ZZU-iPS-AD-MEOX2-001)
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obsolete_Alzheimer's disease
STBCi097-A
(SFC055-04-02)
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STBCi097-B
(SFC055-04-01)
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Alzheimer disease
Last update 25th October 2019
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Provider

Generator Tongji University School of Medicine (TUSM)
Owner Shanghai East Hospital (SHEH)
Derivation country China

External Databases

BioSamples SAMEA4760629
Cellosaurus CVCL_VN40
Wikidata Q98133687

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer Disease
  • Alzheimers Dementia
show more synonyms
Genetic variants

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4760630

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: wangying.csu@126.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Shanghai East Hospital Ethics Committee
Approval number EC.D(BG).016.01.1
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Shanghai East Hospital Ethics Committee
Approval number EC.D(BG).016.01.1
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Source cell origin
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
  • whole blood

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
Smooth Muscle Cell
Ont Id: CL_0000192
Neuron
Ont Id: CL_0000540

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)