BIONi037-A-1

16423 ApoE KO

General

Cell Line

hPSCreg Name
BIONi037-A-1
Alternative name(s)
16423 ApoE KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2022
Notes This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi037-A. The set comprises the following APOE genotypes: • BIONi037-A-1 (APOE KO) • BIONi037-A-2 (APOE 2/2) • BIONi037-A (APOE 3/3) • BIONi037-A-3 (APOE 3/4) • BIONi037-A-4 (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, UKBi011-A and STBci006-A.
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104026332
EBiSC BIONi037-A-1
ECACC 66540581
Cellosaurus CVCL_RF91
CLO CLO_0102746
Wikidata Q54796877

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

Ethics

Also have a look at the ethics information for the parental line BIONi037-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi037-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection refer to BIONi037-A
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Alzheimer disease
Genetic modifications
APOE (target)
Gene knock-out
19q13.32
The ApoE gene is knocked out in this line; deletion of the ApoE gene by insertion of a frame shift in exon 1. The target locus has been mutated.
CRISPR-associated (CRISPR/Cas) System