BIONi037-A-1

16423 ApoE KO

General#

Cell Line

hPSCreg Name BIONi037-A-1
Alternative name(s)
16423 ApoE KO
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th July 2019
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Distributors
Derivation country Denmark

External Databases

BioSamples SAMEA104026332
EBiSC BIONi037-A-1
ECACC 66540581
Cellosaurus CVCL_RF91

General Information

Notes This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi037-A. The set comprises the following APOE genotypes: • BIONi037-A-1 (APOE KO) • BIONi037-A-2 (APOE 2/2) • BIONi037-A (APOE 3/3) • BIONi037-A-3 (APOE 3/4) • BIONi037-A-4 (APOE 4/4) A DNA SNP array revealed no larger chromosomal aberrations to be reported. Other isogenic ApoE cell line cohorts are also available, generated from BIONi010-C, UKBi011-A and STBci006-A.
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

Ethics#

Also have a look at the ethics information for the parental line BIONi037-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi037-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection refer to BIONi037-A
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Alzheimer's disease
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
  • Alzheimer disease
  • Alzheimer's dementia
  • Alzheimer's disease
  • Alzheimer dementia
  • Alzheimers dementia
show more synonyms
Genetic modifications
ApoE (target)
Gene knock-out
19q13.32
The ApoE gene is knocked out in this line; deletion of the ApoE gene by insertion of a frame shift in exon 1. The target locus has been mutated.
CRISPR-associated (CRISPR/Cas) System