GN-JY02-02-011(PSEN1-exon9-KO)
GEMi011-A-1
General
Cell Line |
|
| hPSCreg name | GEMi011-A-1 |
| Cite as: | GEMi011-A-1 |
| Alternative name(s) |
GN-JY02-02-011(PSEN1-exon9-KO)
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
GEMi011-A (GN-IPS-206-1) GEMi022-A-2 (GN-JY02-01-008(PSEN1 c.313T>C)) SIGi001-A-13 (iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11) BIONi037-A-2 (BIONi037-A ApoE2/2 #M10-7) BIONi037-A-3 (BIONi037-A ApoE3/4 #P10-22) BIONi037-A-4 (BIONi037-A ApoE4/4 #I10-53) HMGUi001-A-44 (BIHi043-A-SORL_SNP_Cl-2) BIONi037-A-1 (16423 ApoE KO) WIBRe001-A-58 (WIBR3_VPS13C_FS_Homo_H3-1) WIBRe001-A-59 (WIBR3_VPS13C_A444P_Het_E12-1) WIBRe001-A-60 (WIBR3_VPS13C_A444P_Homo_C8-2) WIBRe001-A-5 (WIBR3_PRKN_X3DEL_F2-5) WIBRe001-A-6 (WIBR3_PRKN_X3DEL_H2-2) WIBRe001-A-7 (WIBR3_SNCA_A30P_A2-3) WIBRe001-A-8 (WIBR3_SNCA_A30P_E1-3) WIBRe001-A-9 (WIBR3_FBXO7_R498X_A7-1) WIBRe001-A-10 (WIBR3_FBXO7_FS_A3-1) WIBRe001-A-11 (WIBR3_PINK1_Q129X_C4-1) WIBRe001-A-12 (WIBR3_PINK1_Q129X_E2-2) WIBRe001-A-13 (WIBR3_PINK1_Q129X_E7-1) WIBRe001-A-15 (WIBR3_DNAJC6_c.801-2 A>G+FS/c.801-2 A>G_G12-2) WIBRe001-A-16 (WIBR3_DNAJC6_FS/FS_H10-1) WIBRe001-A-17 (WIBR3_GBA1_IVS2_G2E) WIBRe001-A-18 (WIBR3_GBA1_IVS2_E10B) WIBRe001-A-19 (WIBR3_SNCA_A53T_1) WIBRe001-A-20 (WIBR3_SNCA_A53T_2) WIBRe001-A-21 (WIBR3_SNCA_A53T_4) WIBRe001-A-22 (WIBR3_LRRK2_G2019S_5_Het) WIBRe001-A-23 (WIBR3_LRRK2_G2019S_6_Het) WIBRe001-A-24 (WIBR3_LRRK2_G2019S_216_Het) WIBRe001-A-25 (WIBR3_LRRK2_G2019S_65_Homo) WIBRe001-A-26 (WIBR3_DJ1_X1-5DEL_2860) WIBRe001-A-27 (WIBR3_DJ1_X1-5DEL_2872) WIBRe001-A-28 (WIBR3_DJ1_X1-5DEL_2876) WIBRe001-A-74 (WIBR3_FBXO7_R498X_A7-1) WIBRe001-A-61 (WIBR3_DJ1_X1-5DEL_Het_2036) WIBRe001-A-62 (WIBR3_DJ1_X1-5DEL_Het_2038) WIBRe001-A-32 (WIBR3_EWT_S1) |
| Last update | 2nd January 2025 |
| User feedback | |
Provider |
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| Generator | Shanghai Gemple Biotech Co.,Ltd (GEM) |
| Owner | Shanghai Gemple Biotech Co.,Ltd (GEM) |
External Databases |
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| BioSamples | SAMEA117551077 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Chinese Han |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | Not available |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
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| BioSamples | SAMEA115947782 |
Ethics
Also have a look at the ethics information for the parental line
GEMi011-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
GEMi011-A.
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| Passage number reprogrammed | P20 |
Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
Protein
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| Protein | |
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Morphology pictures
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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