GN-JY02-01-008(PSEN1 c.313T>C)
GEMi022-A-2
General
Cell Line |
|
| hPSCreg name | GEMi022-A-2 |
| Cite as: | GEMi022-A-2 (RRID:CVCL_E8Y1) |
| Alternative name(s) |
GN-JY02-01-008(PSEN1 c.313T>C)
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
GEMi022-A (GN-IPS-217-1) GEMi011-A-1 (GN-JY02-02-011(PSEN1-exon9-KO)) GEMi022-A-1 (GN-JY02-01-001(APP c.2010-2011 inv)) GEMi022-A-3 (GN-JY02-01-012(APP c.2149G>A)) BIONi010-C-2 (BIONi010-C ApoE E3/E3 #H8 P32) BIONi010-C-3 (BIONi010-C ApoE KO #KO30 P30) BIONi010-C-25 (BIONi010-C heterozygous TREM2 KO) BIONi010-C-70 (BIONi010-C with an APOE 2/2 genotype with an additional, homozygous christchurch mutation) BIONi010-C-71 (BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation) BIONi010-C-4 (BIONi010-C ApoE E4/E4 #B44 P27) BIONi010-C-5 (BIONi010-C CD33 E2del #N14 P26) BIONi010-C-6 (BIONi010-C ApoE E2/E2) BIONi010-C-7 (BIONi010-C Trem2 R47H) BIONi010-C-8 (BIONi010-C Trem2 T66M, #Y5-80) BIONi010-C-9 (BIONi010-C CD33 KO) BIONi010-C-17 (BIONi010-C TREM2 KO) CBIGi001-A-11 (IPSC0075, PRKN Y143D/AIW002-02) UCSFi001-A-74 (FUS-P525L HET 2D1) CBIGi001-A-12 (PRKN-V393D/AIW002-02, IPSC0076) UCSFi001-A-75 (FUS-P525L HOM 2D2) CBIGi001-A-13 (IPSC0078, SCARB2-KO/AIW002-02) CBIGi001-A-14 (IPSC0079, SH3GL2-KO/AIW002-02) CBIGi001-A-15 (SNCA A30P/AIW002-02, IPSC0080) CBIGi001-A-16 (IPSC0081, SNCA A53T/AIW002-02) CBIGi001-A-17 (IPSC0082, SNCA E46K/AIW002-02) CBIGi001-A-18 (IPSC0083, SNCA-KO/AIW002-02) CBIGi001-A-19 (IPSC0084, SOD1 G93A/AIW002-02) CBIGi001-A-21 (IPSC0086, SMPD-KO/AIW002-02) CBIGi001-A-22 (TARDBP-A382T/AIW002-02, IPSC0089) CBIGi001-A-23 (TARDBP-G348C/AIW002-02, IPSC0090) WTSIi018-B-18 (Kolf 2.1 J (TARDBP M337V WT/SNV)) CBIGi001-A-24 (TMEM163-KO/AIW002-02, IPSC0096) CBIGi001-A-25 (IPSC0097, TMEM175-KO/AIW002-02) CBIGi001-A-26 (USP19-KO/AIW002-02, IPSC0098) CBIGi001-A-28 (IPSC0100, ASAH1-KO/AIW002-02) CBIGi001-A-29 (ATAXIN3-KO/AIW002-02, IPSC0101) CBIGi001-A-30 (BIN3-KO/AIW002-02, IPSC0102) CBIGi001-A-31 (IPSC0103, C9ORF72-KO/AIW002-02) |
| Last update | 19th December 2024 |
| User feedback | |
Provider |
|
| Generator | Shanghai Gemple Biotech Co.,Ltd (GEM) |
| Owner | Shanghai Gemple Biotech Co.,Ltd (GEM) |
External Databases |
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| BioSamples | SAMEA116144572 |
| Cellosaurus | CVCL_E8Y1 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Chinese Han |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | Not available |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA115963429 |
Ethics
Also have a look at the ethics information for the parental line
GEMi022-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
GEMi022-A.
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| Passage number reprogrammed | P20 |
Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
Protein
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| Protein | |
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Morphology pictures
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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