GN-JY02-01-001(APP c.2010-2011 inv)
GEMi022-A-1
General
Cell Line |
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hPSCreg name | GEMi022-A-1 |
Cite as: | GEMi022-A-1 |
Alternative name(s) |
GN-JY02-01-001(APP c.2010-2011 inv)
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Last update | 13th September 2024 |
User feedback | |
Provider |
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Generator | Shanghai Gemple Biotech Co.,Ltd (GEM) |
Owner | Shanghai Gemple Biotech Co.,Ltd (GEM) |
External Databases |
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BioSamples | SAMEA116043122 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | male |
Ethnicity | Chinese Han |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Family history | Not available |
Is the medical history available upon request? | No |
Is clinical information available? | No |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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BioSamples | SAMEA115963429 |
Ethics
Also have a look at the ethics information for the parental line
GEMi022-A
.
Is there an MTA available for the cell line? | No |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
GEMi022-A.
|
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Passage number reprogrammed | P10 |
Reprogramming method |
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Vector type | None |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
Protein
|
Protein | |
Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Microbiology / Virus Screening |
|
HIV 1 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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