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General
Cell Line |
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| hPSCreg name | SCTi003-A-1 |
| Cite as: | SCTi003-A-1 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 21st August 2025 |
| Notes | Genome-edited Human iPSC Line, SCTi003-A-1, APOE e4/e4 (SCTi003-A-1) was generated by CRISPR/Cas9 technology, which converted the endogenous apolipoprotein E (APOE) allele APOE ε3 to APOE ε4, resulting in a homozygous APOE ε4/ε4 genotype. APOE ε4 has one of the highest risk scores for late-onset Alzheimer’s disease (AD) and is associated with increased amyloid-β deposition, neuroinflammation, and impaired lipid metabolism (Ayyubova; Garcia et al.).
This product’s parental human induced pluripotent stem cell (hiPSC) line, Healthy Control Human iPSC Line, Female, SCTi003-A, is a well-characterized control line derived from peripheral blood mononuclear cells (PBMCs) from a 48-year-old healthy donor originally heterozygous for APOE ε3/ε4. Targeted gene modifications were confirmed by Sanger sequencing. Post-editing, extensive quality control procedures were undertaken in the manufacturing process for SCTi003-A-1 to ensure optimal product performance and reproducibility. SCTi003-A-1 is karyotypically stable, expresses markers of the undifferentiated state, and remains capable of directed differentiation into all three germ layers, including neural lineage cells relevant for Alzheimer’s disease research. This genome-edited hiPSC line, along with its parental hiPSC line, provides a genetically defined tool for modeling APOE-related mechanisms in Alzheimer’s disease and for preclinical evaluation of targeted therapeutics. SCTi003-A-1 is manufactured with mTeSRTM Plus (Catalog #100-0276) and is compatible with STEMdiffTM cell culture media products, allowing for standardized high-quality maintenance and differentiation to various cell types, such as neurons, astrocytes, and microglia. Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols. |
| User feedback | |
Provider |
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| Generator |
STEMCELL Technologies Inc. (SCT)
Contact:
STEMCELL Technologies Inc. (SCT) |
| Owner | STEMCELL Technologies Inc. (SCT) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA119557645 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
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| Subclone of | |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SCTi003-A.
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