General

iPSC Line
hPSCreg name	WIBRe001-A-25
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	WIBRe001-A-25 (RRID:CVCL_C7VJ)
Alternative name(s)	WIBR3_LRRK2_G2019S_65_Homo
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	WIBRe001-A-22 (WIBR3_LRRK2_G2019S_5_Het) WIBRe001-A-23 (WIBR3_LRRK2_G2019S_6_Het) WIBRe001-A-24 (WIBR3_LRRK2_G2019S_216_Het) WIBRe001-A-40 (WIBR3_LRRK2_G2019S_2093_Het) WIBRe001-A (WIBR38, WIBR3) WIBRe001-A-58 (WIBR3_VPS13C_FS_Homo_H3-1) WIBRe001-A-59 (WIBR3_VPS13C_A444P_Het_E12-1) WIBRe001-A-60 (WIBR3_VPS13C_A444P_Homo_C8-2) WIBRe001-A-5 (WIBR3_PRKN_X3DEL_F2-5) WIBRe001-A-6 (WIBR3_PRKN_X3DEL_H2-2) WIBRe001-A-7 (WIBR3_SNCA_A30P_A2-3) WIBRe001-A-8 (WIBR3_SNCA_A30P_E1-3) WIBRe001-A-9 (WIBR3_FBXO7_R498X_A7-1) WIBRe001-A-10 (WIBR3_FBXO7_FS_A3-1) WIBRe001-A-11 (WIBR3_PINK1_Q129X_C4-1) WIBRe001-A-12 (WIBR3_PINK1_Q129X_E2-2) WIBRe001-A-13 (WIBR3_PINK1_Q129X_E7-1) WIBRe001-A-15 (WIBR3_DNAJC6_c.801-2 A>G+FS/c.801-2 A>G_G12-2) WIBRe001-A-16 (WIBR3_DNAJC6_FS/FS_H10-1) WIBRe001-A-17 (WIBR3_GBA1_IVS2_G2E) WIBRe001-A-18 (WIBR3_GBA1_IVS2_E10B) WIBRe001-A-19 (WIBR3_SNCA_A53T_1) WIBRe001-A-20 (WIBR3_SNCA_A53T_2) WIBRe001-A-21 (WIBR3_SNCA_A53T_4) WIBRe001-A-26 (WIBR3_DJ1_X1-5DEL_2860) WIBRe001-A-27 (WIBR3_DJ1_X1-5DEL_2872) WIBRe001-A-28 (WIBR3_DJ1_X1-5DEL_2876) WIBRe001-A-74 (WIBR3_FBXO7_R498X_A7-1) WIBRe001-A-61 (WIBR3_DJ1_X1-5DEL_Het_2036) WIBRe001-A-62 (WIBR3_DJ1_X1-5DEL_Het_2038) WIBRe001-A-32 (WIBR3_EWT_S1) WIBRe001-A-63 (WIBR3_DJ1_X1-5DEL_Het_2046) WIBRe001-A-33 (WIBR3_EWT_S2) WIBRe001-A-64 (WIBR3_DJ1_X1-5DEL_Het_2051) WIBRe001-A-34 (WIBR3_EWT_S3) WIBRe001-A-65 (WIBR3_DJ1_X1-5DEL_Het_2067) WIBRe001-A-35 (WIBR3_EWT_S4) WIBRe001-A-36 (WIBR3_EWT_S5) WIBRe001-A-66 (WIBR3_DJ1_X1-5DEL_6235) WIBRe001-A-37 (WIBR3_EWT_S6) WIBRe001-A-67 (WIBR3_DJ1_X1-5DEL_6348) WIBRe001-A-38 (WIBR3_EWT_S7) WIBRe001-A-68 (WIBR3_DJ1_X1-5DEL_6390) WIBRe001-A-39 (WIBR3_EWT_S8) WIBRe001-A-75 (WIBR3_SNCA_A30P_A2-3) WIBRe001-A-85 (WIBR3_ATP13A2_FS_Homo_ 6_2) WIBRe001-A-76 (WIBR3_SNCA_A30P_E1-3) WIBRe001-A-77 (WIBR3_FBXO7_FS_A3-1) WIBRe001-A-78 (WIBR3_PINK1_Q129X_C4-1) WIBRe001-A-79 (WIBR3_PINK1_Q129X_E2-2)
Last update	14th December 2024
Notes	Internal experimental control(s) were generated undergoing the same experimental procedure and are registered as WIBRe001-A-35, WIBRe001-A-36, WIBRe001-A-37, WIBRe001-A-38 and WIBRe001-A-39.
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Provider
Generator	Albert Einstein College of Medicine (AECM)
Distributors	WiCell Research Institute - National Stem Cell Bank
External iPSC Databases
BioSamples	SAMEA114404499
Cellosaurus	CVCL_C7VJ
Wikidata	Q123033893
General iPSC Information
Publications	Busquets O et al. iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease. bioRxiv : the preprint server for biology. 2025 Mar 9;.
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed
Subclone of	WIBRe001-A

iPSC Genetic Modification

Disease/phenotype related modifications

Parkinson Disease Synonyms Parkinson Disease Parkinson's disease Parkinson's Disease Genetic modifications LRRK2 leucine rich repeat kinase 2 (target) Type of iPSC Modification Isogenic modification Chromosome location chr12:40340400 Nucleotide sequence variant in HGVS format NM_198578.4:c.6055G>A/c.6055G>A Protein sequence variant in HGVS format NP_940980.4, p.G2019S/p.G2019S Is the modification homozygous or heterozygous? Homozygous

Donor Information

General Donor Information
Sex	female
Ethnicity	unknown
Phenotype and Disease related information (Donor)
Diseases	No disease was diagnosed.
Family history	no
Is the medical history available upon request?	no
Is clinical information available?	no
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No
External Databases (Donor)
BioSamples	SAMEA104129930

Ethics

Also have a look at the ethics information for the parental line WIBRe001-A .

hESC Derivation

The source cell information can be found in the parental cell line WIBRe001-A.

iPSC Culture Conditions

Medium

Other medium: Base medium: DMEM/F12 Main protein source: Fetal bovine serum Serum concentration: 15 % thawing-of-hpscs-grown-on-mefs-b4mnqu5e.pdf freezing-of-hpscs-grown-on-mefs-b4mqqu5w_(1).pdf passaging-of-hpscs-grown-on-mefs-b4mpqu5n.pdf

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes	–
POU5F1 (OCT-4)	Yes	–
SSEA-4	Yes	–

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46XX WIBR3_LRRK2_65.pdf Karyotyping method: Array CGH
Other Genotyping (iPSC Line)