WAe001-A-76

H1-RNF1-/-

General

Cell Line

hPSCreg Name
WAe001-A-76
Alternative name(s)
H1-RNF1-/-
Cell line type Human embryonic stem cell (hESC)
Last update 11th February 2022
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Provider

Generator Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (GIBH)
Owner Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (GIBH)

External Databases

BioSamples SAMEA12038602
CLO CLO_0103776
Cellosaurus CVCL_B5QP
Wikidata Q112041920

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives
Has brother:

External Databases (Donor)

BioSamples SAMEA7767418

Ethics

Also have a look at the ethics information for the parental line WAe001-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe001-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe001-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications not related to a disease
Gene knock-out
RNF1/6p21.32
PCR and Western blot validation for gene knockout
CRISPR-associated (CRISPR/Cas) System