H1_RB1ex3_G3
WAe001-A-10
General
Cell Line |
|
| hPSCreg name | WAe001-A-10 |
| Cite as: | WAe001-A-10 (RRID:CVCL_VE15) |
| Alternative name(s) |
H1_RB1ex3_G3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe001-A-31 (H1_RB1ex1_D6) WAe001-A-11 (H1_RB1ex3_G4) RUCDRi002-A-63 (LVIP05-RB1-CS1) WAe001-A (WA01, H1) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe001-A-32 (H1_RB1ex1_E9) WAe001-A-76 (H1-RNF1-/-) WAe001-A-A (H1X-10/H1.2-20) WAe001-A-2B (H1-NSD2-/-) WAe001-A-B (H1X-59/H1.2-6) WAe001-A-C (H1X-10/H1.3-62) WAe001-A-D (H1X-59/H1.3-68) WAe001-A-E (H1X-10/H1.4-4) WAe001-A-F (H1X-59/H1.4-5) WAe001-A-86 (H1.139, H1.1-39) WAe001-A-72 (H1-FTO-Knockout-13) WAe001-A-87 (H1.213, H1.2-13) WAe001-A-G (H1X-10/H1.5-1) WAe001-A-88 (H1.2-124, H1.2124) WAe001-A-1N (H1-FIS1-KO, WA01-FIS1-KO) WAe001-A-89 (H1.3-64, H1.364) WAe001-A-H (H1X-59/H1.5-33) WAe001-A-1O (WA01-MYB-KO, H1-MYB-KO) WAe001-A-77 (H1 RYBP-FLAG-HA KI) WAe001-A-90 (H1.3-191, H1.3191) WAe001-A-I (H1.0-29/H1.1-13) WAe001-A-41 (H1-RNF2-KO-1#) WAe001-A-J (H1.0-102/H1.1-34) WAe001-A-42 (H1-RNF2-KO-2#) WAe001-A-K (H1.0-29/H1.2-31) WAe001-A-1 (H1.1, WA01.1) WAe001-A-L (H1.0-102/H1.2-6) WAe001-A-M (H1.0-29/H1.3-22) WAe001-A-N (H1.0-102/H1.3-90) WAe001-A-91 (H1.4-126, H1.4126) WAe001-A-O (H1.0-29/H1.4-14) WAe001-A-92 (H1.4-143, H1.4143) WAe001-A-P (H1.0-102/H1.4-19) WAe001-A-93 (H1.5-23, H1.523) WAe001-A-78 (H1X10, H1X-10) WAe001-A-94 (H1.528, H1.5-28) WAe001-A-Q (H1.0-29/H1.5-6) WAe001-A-73 (H1-GATAD2B-KO-79#) WAe001-A-R (H1.0-102/H1.5-44) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Universität Duisburg-Essen (UDE)
Contact:
Universitätsklinikum Essen |
| Owner | Universitätsklinikum Essen |
| Distributors | |
External Databases |
|
| Cellosaurus | CVCL_VE15 |
| BioSamples | SAMEA5912509 |
| Wikidata | Q54993537 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
|
| BioSamples | SAMEA7767418 |
Ethics
Also have a look at the ethics information for the parental line
WAe001-A
.
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Consent documents may be obtained from NIH Registry (number 0043), or from WiCell |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| * Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe001-A.
|
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Unknown |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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