WAe001-A-10

H1_RB1ex3_G3

General#

Cell Line

hPSCreg Name WAe001-A-10
Alternative name(s)
H1_RB1ex3_G3
Cell line type Human embryonic stem cell (hESC)
Last update 17th September 2017

Provider

Generator Universität Duisburg-Essen (UDE)
Owner Universitätsklinikum Essen
Distributors Universitätsklinikum Essen

External Databases

BioSamples SAMEA7767418

General Information

Publications
Is the cell line available in principle? Available, without restrictions
Subclone of

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7767418

Ethics#

We are currently revising the public data view for cell lines registered in hPSCreg. This section will be visible again in the next weeks.

hESC Derivation#

Date of derivation 2016-03-19
Embryo stage 0
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique 0
Cell isolation 0
Cell seeding 0

Culture Conditions#

Surface coating Vitronectin
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Unkown
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unkown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Neuron
Ont Id: CL_0000540
In vivo teratoma

Microbiology / Virus Screening

Certificate of Analysis

Is there a certificate of analysis available?
No

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Genetic Modifications (other than for reprogramming)

Disease/phenotype related modifications
Retinoblastoma
Genetic modifications
RB1 (target)
Gene knock-out
13q14.2
Deletion of seven basepairs in RB1 exon 3 on C allele (heterozygous), detected by next-generation amplicon sequencing
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease