H1_RB1ex3_G3
WAe001-A-10
General
Cell Line |
|
| hPSCreg Name | WAe001-A-10 |
| Alternative name(s) |
H1_RB1ex3_G3
|
| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 22nd May 2019 |
| User feedback | |
Provider |
|
| Generator |
Universität Duisburg-Essen (UDE)
Contact:
Universitätsklinikum Essen |
| Owner | Universitätsklinikum Essen |
| Distributors | |
External Databases |
|
| Cellosaurus | CVCL_VE15 |
| BioSamples | SAMEA5912509 |
| CLO | CLO_0102898 |
| Wikidata | Q54993537 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
| All cell lines of this donor's relatives |
Has brother:
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External Databases (Donor) |
|
| BioSamples | SAMEA7767418 |
Ethics
Also have a look at the ethics information for the parental line
WAe001-A
.
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | Consent documents may be obtained from NIH Registry (number 0043), or from WiCell |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe001-A.
|
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Unknown |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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