WAe009-A-13

H9_RB1ex3_G12LS

General

Cell Line

hPSCreg name WAe009-A-13
Cite as:
WAe009-A-13 (RRID:CVCL_UG29)
Alternative name(s)
H9_RB1ex3_G12LS
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 12th June 2019
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Provider

Generator Universität Duisburg-Essen (UDE)
Owner Universitätsklinikum Essen
Distributors
Derivation country Germany

External Databases

Cellosaurus CVCL_UG29
BioSamples SAMEA5912512
Wikidata Q98134726

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • HD
  • Huntington disease
  • Huntington's chorea
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS iPS-Brew XF, human
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
TBXT
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
NES
Unknown
PAX6
Unknown

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Genetic modifications
RB1 (target)
Gene knock-out
13q14
deletion of seven nucleotides in exon 3 of RB1 gene, heterozygous
CRISPR-associated (CRISPR/Cas) System