WAe009-A-69

General#

Cell Line

hPSCreg Name
WAe009-A-69
Cell line type Human embryonic stem cell (hESC)
Last update 23rd August 2021
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Provider

Generator Anzhen Hospital (ANZHEN)
Owner Anzhen Hospital (ANZHEN)
Distributors
Derivation country China

External Databases

BioSamples SAMEA9787090

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics#

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Hypertrophic cardiomyopathy
Synonyms
  • HCM
  • Cardiomyopathy, hypertrophic
  • Enlarged and thickened heart muscle
Genetic modifications
MYH7 (target)
Gene knock-out
14q11.2
CRISPR-associated (CRISPR/Cas) System