WAe009-A-1D

General

Cell Line

hPSCreg name WAe009-A-1D
Cite as:
WAe009-A-1D (RRID:CVCL_D1GD)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A-62
(KCNQ1 KO)
WAe009-A
(WA09, H9)
WAe009-A-1H
(MYL3-KO)
WAe009-A-36
(JPH2-KO)
WAe009-A-1E
(TBX20-KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-59
(dHAND KO)
WAe009-A-58
(COL4A5 heterozygote)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-72
(COL1A2 -/-)
WAe009-A-1P
(IsoHD-30Q-NGN2)
WAe009-A-40
(H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2)
WAe009-A-1Q
(IsoHD-65Q-NGN2)
WAe009-A-1R
(IsoHD-81Q-NGN2)
WAe009-A-60
(H9 iNK5A)
WAe009-A-81
(H9-MSX1-/- cell line)
WAe009-A-77
(H9-GFP)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
Last update 6th October 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Cancer Center,Union Hospital (CCUH)
Owner Cancer Center,Union Hospital (CCUH)
Distributors
Derivation country China

External Databases

BioSamples SAMEA114467815
Cellosaurus CVCL_D1GD
Wikidata Q127384684

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: research
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution The cell is available from the corresponding author upon reasonable request.

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-81
Yes
Morphology pictures
The qRT-PCR results showed that the cells expressed markers such as DPPA4, SOX2, REX-1 and NANOG.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,xx
Passage number: 15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • Romano-Ward Syndrome
  • Long QT Syndrome 1
  • LQT1
Genetic modifications
KCNQ1 (target)
Variant
11p15.5-p15.4
NM_000218.3(KCNQ1):c.1032+2T>C
Homozygous