WA09, H9

General#

Cell Line

hPSCreg Name WAe009-A
Alternative name(s)
WA09, H9
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator WiCell Research Institute (WA)
Distributors
Derivation country United States

External Databases

BioSamples SAMEA7769668
Cellosaurus CVCL_9773
NIHhESC NIHhESC-10-0062

General Information

Publications
Projects
Is the cell line available in principle? Available with restrictions:
Subclones

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA7768918

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
Cell isolation Immunosurgery
Cell seeding Isolated ICM
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
Yes

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
LIF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SSEA-1
No
DNMT3B
Yes
GDF3
Yes
ZFP42 (REX-1)
Yes
GABRB3
Yes
NANOG
Yes
TDGF1
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 30

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)