WAe009-A-89

General

Cell Line

hPSCreg Name
WAe009-A-89
Cell line type Human embryonic stem cell (hESC)
Last update 11th April 2022
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Provider

Generator Anzhen Hospital (ANZHEN)
Owner Anzhen Hospital (ANZHEN)
Distributors
Derivation country China

External Databases

BioSamples SAMEA14029747
CLO CLO_0103893
Cellosaurus CVCL_C1JM

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
/Users/mashuhong/Documents/课题/experiment/projects/TIMP3-k0/ 核型 /Karyotyping.tif
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Sorsby fundus dystrophy
Genetic modifications
Gene knock-out
22q12.3
CRISPR-associated (CRISPR/Cas) System