WAe009-A-74

General

Cell Line

hPSCreg Name
WAe009-A-74
Cell line type Human embryonic stem cell (hESC)
Last update 2nd March 2022
Notes no
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Provider

Generator Shenzhen People's Hospital (SPH)
Owner Shenzhen People's Hospital (SPH)
Distributors
Derivation country China

External Databases

BioSamples SAMEA10772827
CLO CLO_0103571
Cellosaurus CVCL_B3S7
Wikidata Q110433970

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: none
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:

none

Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,xx
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 30

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 30
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Long QT Syndrome 2
none
Synonyms
  • LQT2
  • Long QT Syndrome 2
Genetic modifications
KCNH2 (target)
Variant
7q36.1
NM_000238.3:c.1847 A>G
p.Tyr616Cys
Heterozygous
VCV000067295
None