WAe009-A-74
General
Cell Line |
|
| hPSCreg name | WAe009-A-74 |
| Cite as: | WAe009-A-74 (RRID:CVCL_B3S7) |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-1H (MYL3-KO) WAe009-A-36 (JPH2-KO) WAe009-A-1E (TBX20-KO) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-59 (dHAND KO) WAe009-A-58 (COL4A5 heterozygote) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-72 (COL1A2 -/-) WAe009-A-1P (IsoHD-30Q-NGN2) WAe009-A-40 (H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2) WAe009-A-1Q (IsoHD-65Q-NGN2) WAe009-A-1R (IsoHD-81Q-NGN2) WAe009-A-60 (H9 iNK5A) WAe009-A-81 (H9-MSX1-/- cell line) WAe009-A-77 (H9-GFP) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) |
| Last update | 2nd March 2022 |
| Notes | no |
| User feedback | |
Provider |
|
| Generator |
Shenzhen People's Hospital (SPH)
Contact:
Shenzhen People's Hospital (SPH) |
| Owner | Shenzhen People's Hospital (SPH) |
| Distributors | |
| Derivation country | China |
External Databases |
|
| BioSamples | SAMEA10772827 |
| Cellosaurus | CVCL_B3S7 |
| Wikidata | Q110433970 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: none
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
none |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
|||||
| SSEA-4 |
Yes |
Differentiation Potency
In vivo teratoma
Morphology
In vivo teratoma
Morphology
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
30
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
|
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