General

Cell Line

hPSCreg name WAe009-A-N
Cite as:
WAe009-A-N (RRID:CVCL_C9JB)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-81
(H9-MSX1-/- cell line)
WAe009-A-1H
(MYL3-KO)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-72
(COL1A2 -/-)
WAe009-A-1P
(IsoHD-30Q-NGN2)
WAe009-A-40
(H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2)
WAe009-A-59
(dHAND KO)
WAe009-A-1Q
(IsoHD-65Q-NGN2)
WAe009-A-1R
(IsoHD-81Q-NGN2)
WAe009-A-60
(H9 iNK5A)
WAe009-A-77
(H9-GFP)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
Last update 21st April 2023
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Provider

Generator Henan Provincial Chest Hospital (HPCH)

External Databases

BioSamples SAMEA112945612
Cellosaurus CVCL_C9JB
Wikidata Q123033863

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Non coated
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
Marker Present Absent
mCpG X
OCT4 X
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Human TLR4 biallelic defect can lead to inflammatory bowel disease.
Synonyms
  • IBD
  • Inflammatory Bowel Disease
  • Autoimmune Bowel Disorder
  • inflammatory bowel disease
show more synonyms
Genetic modifications
TLR4 (target)
Gene knock-out
9q33.1
In this clone, Sanger sequencing revealed a 7 bp deletion in one allele and a 8 bp deletion in the other allele.
CRISPR-associated (CRISPR/Cas) System
Genetic modifications not related to a disease
TLR4 (target)
Gene knock-out
9q33.1
CRISPR-associated (CRISPR/Cas) System