WAe009-A-G
General
Cell Line |
|
| hPSCreg name | WAe009-A-G |
| Cite as: | WAe009-A-G (RRID:CVCL_WN07) |
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-40 (H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-60 (H9 iNK5A) WAe009-A-77 (H9-GFP) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-1J (H9::mcherry cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1V (H9-AAVS1-Teton-KrasG12D) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) |
| Last update | 23rd February 2023 |
| User feedback | |
Provider |
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| Generator | Institute For Stem Cell Biology and Regenerative Medicine (INSTEM) |
External Databases |
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| BioSamples | SAMEA112681014 |
| Cellosaurus | CVCL_WN07 |
| Wikidata | Q94318201 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | Yes |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | As listed in SLA (page 2 -4) |
hESC Derivation
|
The source cell information can be found in the parental cell line
WAe009-A.
|
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
Releaser - Stem Cell Technologies
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| Medium |
mTeSR™ 1
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
Morphology pictures
Teratoma assay shpwed differentiation into all three lineages
Method documentation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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