WAe009-A-40

H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2

General

Cell Line

hPSCreg name WAe009-A-40
Cite as:
WAe009-A-40 (RRID:CVCL_WM07)
Alternative name(s)
H9 CBFA2T3-GLIS2, H9 ETO2-GLIS2
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
Donor diseases:
Huntington's disease
WAe009-A-1P
(IsoHD-30Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1Q
(IsoHD-65Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-1R
(IsoHD-81Q-NGN2)
Donor diseases:
Huntington's disease
WAe009-A-12
(H9_RB1ex3_C7)
Donor diseases:
Huntington's disease
WAe009-A-13
(H9_RB1ex3_G12LS)
Donor diseases:
Huntington's disease
WAe009-A-60
(H9 iNK5A)
Donor diseases:
Huntington's disease
WAe009-A-1H
(MYL3-KO)
Donor diseases:
Huntington's disease
WAe009-A-36
(JPH2-KO)
Donor diseases:
Huntington's disease
WAe009-A-1E
(TBX20-KO)
Donor diseases:
Huntington's disease
WAe009-A-59
(dHAND KO)
Donor diseases:
Huntington's disease
WAe009-A-62
(KCNQ1 KO)
Donor diseases:
Huntington's disease
WAe009-A-1G
(ISL1-KO)
Donor diseases:
Huntington's disease
WAe009-A-72
(COL1A2 -/-)
Donor diseases:
Huntington's disease
WAe009-A-81
(H9-MSX1-/- cell line)
Donor diseases:
Huntington's disease
WAe009-A-1I
(H9::GFP cyto-reporter line)
Donor diseases:
Huntington's disease
WAe009-A-1J
(H9::mcherry cyto-reporter line)
Donor diseases:
Huntington's disease
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
Donor diseases:
Huntington's disease
WAe009-A-37
(H9-GSX2-tTA:GFP)
Donor diseases:
Huntington's disease
WAe009-A-58
(COL4A5 heterozygote)
Donor diseases:
Huntington's disease
Last update 20th April 2022
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Provider

Generator Centre for Genomics and Oncological Research (GENYO)

External Databases

BioSamples SAMEA7124963
Cellosaurus CVCL_WM07
Wikidata Q93937305

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • HD
  • Huntington disease
  • Huntington's chorea
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • AML
  • Acute myelogenous leukemia
  • Acute myeloblastic leukemia
  • Acute myelocytic leukemia
  • Acute myeloid leukaemia
  • Acute myelogenous leukaemia
  • Acute myelocytic leukaemia
  • Acute myeloblastic leukaemia
show more synonyms
Genetic modifications
Transgene expression
inv 16
The cell line WAe009-A-40 was generated by lentiviral transduction of the cell line WAe009-A for the expression of the transgene ETO2-GLIS2 (CBFA2T3-GLIS2). In patients, the transgene ETO2-GLIS2 is generated by inversion of chromosome 16 (inv16). The transgene ETO2-GLIS2 from a patient with acute myeloid leukemia were subcloned in a lentiviral vector and was used for lentiviral particles generation.
Viral
Lentivirus