WAe009-A-60

H9 iNK5A

General

Cell Line

hPSCreg Name
WAe009-A-60
Alternative name(s)
H9 iNK5A
Cell line type Human embryonic stem cell (hESC)
Last update 20th April 2022
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Provider

Generator Centre for Genomics and Oncological Research (GENYO)

External Databases

BioSamples SAMEA8944454
Cellosaurus CVCL_A8DI
CLO CLO_0103329
Wikidata Q107117435

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46, XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? Yes
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 51
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
childhood acute myeloid leukemia
Synonyms
  • childhood acute myelogenous leukemia
  • childhood acute myeloid leukaemia
  • acute myeloid leukemia (AML)
  • childhood acute myeloblastic leukemia
  • pediatric acute myeloid leukemia
  • childhood acute myeloid leukemia
  • paediatric acute myeloid leukaemia
  • pediatric AML
  • pediatric acute myelocytic leukemia
  • childhood acute granulocytic leukemia
  • pediatric acute myeloblastic leukemia
  • childhood AML
  • pediatric acute myelogenous leukemia
  • childhood acute myelocytic leukemia
  • acute myeloid leukemia of childhood
show more synonyms
Genetic modifications
Gene knock-in
19q13.4-qter
The cell line WAe009-A-60 was generated by homologous recombination of the cell line WAe009-A for the expression of the transgene NUP98-KDM5A. In patients, the transgene NUP98-KDM5A is generated by the translocation of chromosome 11 and 12 giving the t(11;12)(p15;p13).
Homologous Recombination
Genetic modifications not related to a disease
Gene knock-in
19q13.4-qter
Homologous Recombination