WAe009-A-24

General#

Cell Line

hPSCreg Name WAe009-A-24
Cell line type Human embryonic stem cell (hESC)
Last update 4th November 2019
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Provider

Generator Monash Institute of Pharmaceutical Sciences (MIPS)
Owner Monash Institute of Pharmaceutical Sciences (MIPS)
Derivation country Australia

External Databases

BioSamples SAMEA6324522

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity human

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7768918

Ethics#

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No

hESC Derivation#

Date of derivation 2019-09-30
Supernumerary embryos from IVF treatment?
No
Other hESC source: The line is derived from WAe009-A
PGD Embryo?
No

Culture Conditions#

Surface coating Matrigel/Geltrex

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
CX3CR1 (target)
Gene knock-in
3p22.2
This line is engineered to include a genetic reporter of CX3CR1 expression which results in expression of tdTomato and nanoluciferase in series with CX3CR1 expression. The construct also includes a neomycin resistance cassette flanked by FRT sites. One allele of the CX3CR1 gene contains the following construct targeted to the stop codon: IRES-tdTomato-T2A-Nanoluc-pA-FRT-neo-FRT.