WAe009-A-24

General

Cell Line

hPSCreg name WAe009-A-24
Cite as:
WAe009-A-24 (RRID:CVCL_YN82)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
WAe009-A-72
(COL1A2 -/-)
WAe009-A-58
(COL4A5 heterozygote)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-62
(KCNQ1 KO)
Last update 4th November 2019
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Provider

Generator Monash Institute of Pharmaceutical Sciences (MIPS)
Owner Monash Institute of Pharmaceutical Sciences (MIPS)
Derivation country Australia

External Databases

BioSamples SAMEA6324522
Cellosaurus CVCL_YN82
Wikidata Q98134748

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
CX3CR1 (target)
Gene knock-in
3p22.2
This line is engineered to include a genetic reporter of CX3CR1 expression which results in expression of tdTomato and nanoluciferase in series with CX3CR1 expression. The construct also includes a neomycin resistance cassette flanked by FRT sites. One allele of the CX3CR1 gene contains the following construct targeted to the stop codon: IRES-tdTomato-T2A-Nanoluc-pA-FRT-neo-FRT.