WAe009-A-47

General

Cell Line

hPSCreg name WAe009-A-47
Cite as:
WAe009-A-47 (RRID:CVCL_A4UB)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-58
(COL4A5 heterozygote)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
Last update 2nd November 2020
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Provider

Generator Peking University Third Hospital Department of Cardiology (PUHT)
Owner Peking University Third Hospital Department of Cardiology (PUHT)
Distributors
Derivation country China

External Databases

BioSamples SAMEA7679543
Cellosaurus CVCL_A4UB
Wikidata Q107117420

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Is there an MTA available for the cell line? No
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 20
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • cytochrome-C oxidase deficiency
  • cytochrome-c oxidase deficiency disease
  • isolated COX deficiency
  • isolated mitochondrial respiratory chain complex IV deficiency
  • mitochondrial complex IV deficiency
  • COX deficiency
  • Complex 4 mitochondrial respiratory chain deficiency
  • Complex IV deficiency
  • Cox deficiency
  • cytochrome C oxidase deficiency
  • deficiency of mitochondrial respiratory chain complex4
  • isolated cytochrome C oxidase deficiency
  • mitochondrial Complex 4 deficiency
  • Cytochrome C Oxidase Deficiency
show more synonyms
Genetic modifications
COX6A2 (target)
Gene knock-out
16p11.2
Exon 1, 2bp insertion , insertion sequence"CT", causing frameshift mutation and a premature stop codon
CRISPR-associated (CRISPR/Cas) System