General#

Cell Line

hPSCreg Name WAe009-A-20
Cell line type Human embryonic stem cell (hESC)
Last update 22nd May 2019
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Provider

Generator VIB - KU Leuven Center for Brain and Disease Research (VIBCBD)

External Databases

BioSamples SAMEA5728497
Cellosaurus CVCL_WJ42

General Information

Projects
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity human

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7768918

Ethics#

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation#

Date of derivation 1998-01-01
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
Separation of research and IVF treatment?
Yes
PGD Embryo?
No
ZP removal technique Chemical
Cell isolation Immunosurgery
Cell seeding Embryo

Culture Conditions#

Medium Other medium:
Base medium: Essential 8 flex kit
Main protein source:
Serum concentration: %

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Transgene expression
19q13-qter
Doxycycline inducible dCAS9 fused with VP64-P16-RTA along with GFP and rtTA (3rd generation) driven by CAGS promoter. Transgene delivered by electroporation and inserted in the Adeno-associated virus integration site 1 (AAVS1).
Homologous Recombination