H9::mcherry cyto-reporter line
WAe009-A-1J
General
Cell Line |
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| hPSCreg name | WAe009-A-1J |
| Cite as: | WAe009-A-1J |
| Alternative name(s) |
H9::mcherry cyto-reporter line
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
WAe009-A (WA09, H9) WAe009-A-77 (H9-GFP) WAe009-A-1H (MYL3-KO) WAe009-A-1I (H9::GFP cyto-reporter line) WAe009-A-78 (TBX18-KO) WAe009-A-7 (H9-mHOXA9) WAe009-A-R (H9-NRL-GP, WA09 NRL+/eGFP) WAe009-A-36 (JPH2-KO) WAe009-A-12 (H9_RB1ex3_C7) WAe009-A-13 (H9_RB1ex3_G12LS) WAe009-A-1E (TBX20-KO) WAe009-A-37 (H9-GSX2-tTA:GFP) WAe009-A-99 (dCas9-p300 H9 21) WAe009-A-1V (H9-AAVS1-Teton-KrasG12D) WAe009-A-62 (KCNQ1 KO) WAe009-A-1G (ISL1-KO) WAe009-A-90 (H9_LCCS1) WAe009-A-72 (COL1A2 -/-) WAe009-A-58 (COL4A5 heterozygote) |
| Last update | 26th October 2023 |
| User feedback | |
Provider |
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| Generator | Memorial Sloan Kettering Cancer Center (MSK) |
External Databases |
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| BioSamples | SAMEA114549740 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | N/A |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Family history | NO |
| Is the medical history available upon request? | NO |
| Is clinical information available? | NO |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA7768918 |
Ethics
Also have a look at the ethics information for the parental line
WAe009-A
.
| Is there an MTA available for the cell line? | No |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hESC Derivation
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The source cell information can be found in the parental cell line
WAe009-A.
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Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
Morphology pictures
Differentiation Potency
In vitro directed differentiation
Protocol or reference
NC differentiation.jpg
Figure 5. Vagal and sacral NC exhibits distinct behavior in vitro (A) Schematic drawing of experimental design. RFP-tagged hPSC line was used for vagal lineages and GFP-tagged line for sacral lineages. (B) qRT-PCR of SOX10 and HOX genes to confirm the VNC and SNC identity. n = 6 biological replicates (C) Invasion assay of VNC and SNC. Cells crossed the membrane are visualized (left) and quantified using plater reader (right). Scale bars, 50 mm. n = 3 biological replicates. (D) Migration assay of VNC and SNC on PO/LM/FN surface. Cells migrated out of spherical aggregate were imaged (left) and migration distance were quantified (right). Scale bars, 500 mm. n = 3 biological replicates. (E) 3D Matrigel embedded migration assay of co-cultured VNC and SNC. Fluorescence image of cells at 24 (upper) and 96 h (lower), show self-sorting activity. Scale bars, 500 mm. (F) Co-cultured VNC (red) and SNC (green) cells undergoing ENS differentiation with replating at day 10. Scale bars, 100 mm.
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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