WAe009-A-1X

H9 ALK CRISPR F1174L

General

Cell Line

hPSCreg name WAe009-A-1X
Cite as:
WAe009-A-1X
Alternative name(s)
H9 ALK CRISPR F1174L
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 15th August 2024
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Provider

Generator STEMCELL Technologies Inc. (SCT)

External Databases

BioSamples SAMEA115892179

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
POU5F1
No
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
Nestin
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal
Passage number: 53
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes

Genetic Modification

Disease/phenotype related modifications
Synonyms
  • neural Crest tumor, malignant
  • neuroblastoma
  • neuroblastoma (Schwannian Stroma-poor)
  • neuroblastoma, malignant
  • NB
show more synonyms
Genetic modifications
ALK (target)
Variant
2p23.2-p23.1
NC_000002.12:g.29220831A>G
NP_004295.2:p.Phe1174Leu
Heterozygous
RCV000201883.2
CRISPR/Cas9 induced knock-in of the ALK F1174L mutation
Genetic modifications not related to a disease
ALK (target)
Isogenic modification
2p23.2-p23.1
NC_000002.12:g.29220820C>T
Homozygous
CRISPR/Cas9 silent mutation
Mutated
ALK (target)
Isogenic modification
2p23.2-p23.1
NC_000002.12:g.2922081G>A
Homozygous
CRISPR/Cas9 silent mutation
Mutated