WAe009-A-76

General

Cell Line

hPSCreg name WAe009-A-76
Cite as:
WAe009-A-76 (RRID:CVCL_B5QI)
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
MRIi001-A-1
(C6-AAVS1-iCasRx)
ICGi021-A-1
(K6-4fpCyto-13)
MRIi003-A-8
(HK-AAVS1-CAG-eGFP-homo)
ICGi021-A-2
(K6-4fpCyto-16)
ICGi021-A-3
(K6-4fpCyto-19)
UCSFi001-A-12
(AICS-0036-006)
MRIi003-A-7
(HK-AAVS1-iCasRx)
UCSFi001-A-23
(AICS-0054-091)
BIHi001-A-1
(iBCRT Cas9v1-3G-Kl.16)
UCSFi001-A-36
(AICS-0036-028)
CRMi003-A-3
(Proliving, GMNN-mScarletI Reporter)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-58
(COL4A5 heterozygote)
WAe009-A-1P
(IsoHD-30Q-NGN2)
WAe009-A-59
(dHAND KO)
WAe009-A-1Q
(IsoHD-65Q-NGN2)
WAe009-A-1R
(IsoHD-81Q-NGN2)
WAe009-A-1W
(H9 EF1A-mCherry-noPuro 803_AAVS-iSHH #3)
WAe009-A-60
(H9 iNK5A)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
Last update 11th November 2021
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Provider

Generator IMP - Research Institute of Molecular Pathology (IMP)

External Databases

BioSamples SAMEA10839123
Cellosaurus CVCL_B5QI
Wikidata Q112041921

General Information

Projects
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A .

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
AAVS1 (target)
Gene knock-in
19q13
CAGGS:loxP-STOP-loxp_mCherrynls-T2A-Puro Plasmids introduced using electroporation
TALEN
Bestrophin1 (target)
Gene knock-in
11q12.3
Best1 C-terminus: T2A-H2BVenus-p2A-CreERT Plasmids introduced using electroporation
CRISPR-associated (CRISPR/Cas) System