WAe009-A-76

General#

Cell Line

hPSCreg Name
WAe009-A-76
Cell line type Human embryonic stem cell (hESC)
Last update 11th November 2021
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Provider

Generator IMP - Research Institute of Molecular Pathology (IMP)

External Databases

BioSamples SAMEA10839123
CLO CLO_0103574

General Information

Projects
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics#

Also have a look at the ethics information for the parental line WAe009-A .

hESC Derivation#

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line WAe009-A

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
AAVS1 (target)
Gene knock-in
19q13
CAGGS:loxP-STOP-loxp_mCherrynls-T2A-Puro Plasmids introduced using electroporation
TALEN
Bestrophin1 (target)
Gene knock-in
11q12.3
Best1 C-terminus: T2A-H2BVenus-p2A-CreERT Plasmids introduced using electroporation
CRISPR-associated (CRISPR/Cas) System