K6-4fpCyto-19
ICGi021-A-3
General
Cell Line |
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| hPSCreg name | ICGi021-A-3 |
| Cite as: | ICGi021-A-3 |
| Alternative name(s) |
K6-4fpCyto-19
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
ICGi021-A-1 (K6-4fpCyto-13) ICGi021-A-2 (K6-4fpCyto-16) ICGi021-A (K6-4f) MRIi001-A-1 (C6-AAVS1-iCasRx) UCSFi001-A-12 (AICS-0036-006) CRMi003-A-3 (Proliving, GMNN-mScarletI Reporter) MRIi003-A-7 (HK-AAVS1-iCasRx) MRIi003-A-8 (HK-AAVS1-CAG-eGFP-homo) UCSFi001-A-23 (AICS-0054-091) UCSFi001-A-36 (AICS-0036-028) BIHi001-A-1 (iBCRT Cas9v1-3G-Kl.16) |
| Last update | 18th June 2021 |
| Notes | Healthy human iPSC clone with doxycycline-inducible expression of cytosolic Grx1-roGFP2 |
| User feedback | |
Provider |
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| Generator | Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG) |
| Derivation country | Russia |
External Databases |
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| BioSamples | SAMEA9232931 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: Any
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ncbi.nlm.nih.gov/sra/?term=SRR11413028 No disease associated mutation found |
External Databases (Donor) |
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| BioSamples | SAMEA6983569 |
Ethics
Also have a look at the ethics information for the parental line
ICGi021-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
ICGi021-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin | ||||||||||||||||||
| Feeder cells |
MEF cell Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO_0004040 |
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| Passage method |
Enzymatically
TrypLE
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| O2 Concentration | 20 % | ||||||||||||||||||
| CO2 Concentration | 5 % | ||||||||||||||||||
| Medium |
Other medium:
Base medium: Knock-out DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| SSEA-4 |
Yes |
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| Alkaline Phosphatase |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
Morphology
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| Tubulin, beta 3 |
Yes |
| Neurofilament 200 |
Yes |
Morphology
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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| Genetic modifications not related to a disease |
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