CRMi003-A-3

Proliving, GMNN-mScarletI Reporter

General

Cell Line

hPSCreg Name
CRMi003-A-3
Alternative name(s)
Proliving, GMNN-mScarletI Reporter
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 3rd August 2022
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Provider

Generator Leiden University Medical Center (LUMC)
Owner LUMC hiPSC core facility
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA110425584
CLO CLO_0103943
Cellosaurus CVCL_C1P1
Wikidata Q114311268

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity unknown

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history unknown
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7776418

Ethics

Also have a look at the ethics information for the parental line CRMi003-A .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line CRMi003-A.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Colony Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex medium
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 32
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
AAVS1 (target)
Gene knock-in
19q13
We introduced a mScarletI-GMNN(1-110) cell cycle reporter into the AAVS1 locus of a PAX3-Venus hiPSC line already containing a doxycycline-inducible H2B-mTurquoise2 (LUMC041-A-2). The hiPSC reporter line generated not only allowed tracking of living cells but also analysis of specific cell cycle stages in real time
CRISPR-associated (CRISPR/Cas) System