Proliving, GMNN-mScarletI Reporter
CRMi003-A-3
General
Cell Line |
|
| hPSCreg name | CRMi003-A-3 |
| Cite as: | CRMi003-A-3 (RRID:CVCL_C1P1) |
| Alternative name(s) |
Proliving, GMNN-mScarletI Reporter
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CRMi003-A (NCRM-1, NL-1) UCSFi001-A-12 (AICS-0036-006) MRIi003-A-7 (HK-AAVS1-iCasRx) MRIi003-A-8 (HK-AAVS1-CAG-eGFP-homo) UCSFi001-A-23 (AICS-0054-091) UCSFi001-A-36 (AICS-0036-028) BIHi001-A-1 (iBCRT Cas9v1-3G-Kl.16) MRIi001-A-1 (C6-AAVS1-iCasRx) ICGi021-A-1 (K6-4fpCyto-13) ICGi021-A-2 (K6-4fpCyto-16) ICGi021-A-3 (K6-4fpCyto-19) |
| Last update | 3rd August 2022 |
| User feedback | |
Provider |
|
| Generator | Leiden University Medical Center (LUMC) |
| Owner | LUMC hiPSC core facility |
| Distributors | |
| Derivation country | Netherlands |
External Databases |
|
| BioSamples | SAMEA110425584 |
| Cellosaurus | CVCL_C1P1 |
| Wikidata | Q114311268 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
| Ethnicity | unknown |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | unknown |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA7776418 |
Ethics
Also have a look at the ethics information for the parental line
CRMi003-A
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
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The source cell information can be found in the parental cell line
CRMi003-A.
|
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Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
Unknown |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Selection criteria for clones | Colony Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFlex medium
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY
Passage number: 32
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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