ICGi015-B-1

m6.7pCyto-17

General#

Cell Line

hPSCreg Name
ICGi015-B-1
Alternative name(s)
m6.7pCyto-17
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th July 2021
Notes Parkinson's disease patient-specific iPSC clone with doxycycline-inducible expression of cytosolic Grx1-roGFP2
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Provider

Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Derivation country Russia

External Databases

BioSamples SAMEA9232933
CLO CLO_0103417

General Information

* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Any
Research: allowed
Clinical: not allowed
Commercial: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6098112

Ethics#

Also have a look at the ethics information for the parental line ICGi015-B .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

The source cell information can be found in the parental cell line ICGi015-B.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells MEF cell
Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO_0004040
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock-out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Supplements
GlutaMAX 2 mM
NEAA 0.1 mM
Penicillin-Streptomycin 100 U/ml
2-mercaptoethanol 0.1 mM
rhFGF basic 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK-18
Yes
GATA6
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
aSMA
Yes
Collagen type I
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Tubulin, beta 3
Yes
Neurofilament 200
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Parkinson's Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
Genetic modifications not related to a disease
AAVS1 (target)
Transgene expression
19q13
Transgenes of glutathione redox sensor (cytosolic) cyto_Grx1-roGFP2 and tetracycline-transactivator (rtTA) in AAVS1 locus were detected by PCR.
CRISPR-associated (CRISPR/Cas) System
puro in.TIF
cyto Grx1-roGFP2 transgene detection
neo in.TIF
rtTA transgene detection