AST18-6A
EDi001-B-4
General
Cell Line |
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hPSCreg name | EDi001-B-4 |
Cite as: | EDi001-B-4 (RRID:CVCL_A1XS) |
Alternative name(s) |
AST18-6A
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
EDi001-A-2 (AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-3 (AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A-4 (AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi001-A (AST22, AST23, SAMEA3319992) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: Parkinson disease EDi008-B (G51D-4, EDINi008-B, EDIi008-B, SAMEA3174606) Donor's gene variants: SNCA, SNCA, SNCA, SNCA Donor diseases: Parkinson disease STBCi004-B-1 (SFC832-03-06 LRRK2WT/WT C47) Donor's gene variants: LRRK2 Donor diseases: Parkinson disease HIHDNDi001-B (A30P-4, SNCA4, Tue_020_B) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: autosomal dominant Parkinson disease 1 HIHDNDi001-A (A30P-3, SNCA3, Tue_020_A) Donor's gene variants: SNCA, SNCA, SNCA Donor diseases: autosomal dominant Parkinson disease 1 |
Last update | 14th May 2022 |
User feedback | |
Provider |
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Generator | University of Edinburgh (ED) |
Distributors | |
External Databases |
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BioSamples | SAMEA7111753 |
Cellosaurus | CVCL_A1XS |
Wikidata | Q105506819 |
General Information |
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Publications |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Family history | Strong family history of Parkinson’s disease due to autosomal dominant inheritance of SNCA triplication |
Is the medical history available upon request? | Y Mov Disord. 2011 Sep;26(11):2134-6. doi: 10.1002/mds.23776 |
Donor Relations |
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Other cell lines of this donor | |
All cell lines of this donor's relatives |
Has daughter:
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External Databases (Donor) |
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BioSamples | SAMEA3319991 |
Ethics
Also have a look at the ethics information for the parental line
EDi001-B
.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
EDi001-B.
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Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
Unknown |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Laminin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: StemMACS™ iPS-Brew XF
Main protein source: Serum concentration: % |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
No gross chromosomal abnormalities.
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
Genetic Modification
Disease/phenotype related modifications |
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