General#

Cell Line
hPSCreg Name A standardised name that was automatically generated based on the cell line information. see Naming Tool	LCSBi008-A-1
Alternative name(s)	delP GC13, DJ-1-delP GC13
Cell line type	Human induced pluripotent stem cell (hiPSC)
Last update	4th April 2022
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Provider
Generator	Luxembourg Centre for Systems Biomedicine (LCSB)
External Databases
BioSamples	SAMEA13914317
General Information
Publications	Mencke P et al. Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1). Stem cell research. 2022 May 17;62:102815.
* Is the cell line readily obtainable for third parties?	No
Subclone of	LCSBi008-A

Donor Information#

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Parkinson's Disease Parkinson's Disease in the OLS Synonyms Parkinson Disease Parkinson's disease Parkinson's Disease
External Databases (Donor)
BioSamples	SAMEA13793663

Ethics#

Also have a look at the ethics information for the parental line LCSBi008-A .

Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General
The source cell information can be found in the parental cell line LCSBi008-A.
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions#

Medium

Essential 8™

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
SOX2	Yes
POU5F1 (OCT-4)	Yes
DNMT3B	Yes

Morphology

Morphology pictures

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY
Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications	Parkinson's Disease Parkinson's Disease in the OLS Synonyms Parkinson Disease Parkinson's disease Parkinson's Disease Genetic modifications PARK7 (target) Type of modification Isogenic modification Is the modification homozygous or heterozygous? Homozygous How has the target locus been modified? Repaired
Genetic modifications not related to a disease