Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)


We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Mencke P, Hanss Z, Jarazo J, Massart F, Rybicki A, Petkovski E, Glaab E, Boussaad I, Bonifati V, Christian Schwamborn J, Mandemakers W, Krüger R
Journal Stem cell research
Publication Date 2022 Jul;62:102815
PubMed 35617842
DOI 10.1016/j.scr.2022.102815

Research Projects

Cell Lines