Generation of isogenic control DJ-1-delP GC13 for the genetic Parkinson's disease-patient derived iPSC line DJ-1-delP (LCSBi008-A-1)
We describe the generation of an isogenic control cell line DJ-1-delP GC13 from an induced pluripotent stem cell (iPSC) line DJ-1-delP LCSBi008-A that was derived from fibroblasts obtained from a Parkinson's disease (PD) patient. Using CRISPR/Cas9 technology, we corrected the disease causing c.471_473delGCC homozygous mutation in the PARK7 gene leading to p.158P deletion in the encoded protein DJ-1. The generated isogenic pair will be used for phenotypic analysis of PD-patient derived neurons and astrocytes. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
|Authors||Mencke P, Hanss Z, Jarazo J, Massart F, Rybicki A, Petkovski E, Glaab E, Boussaad I, Bonifati V, Christian Schwamborn J, Mandemakers W, Krüger R|
|Journal||Stem cell research|
|Publication Date||2022 Jul;62:102815|