SUSMi005-A-4

SNCA3X 3KO C1, SNCA3X 3KO C2

General

Cell Line

hPSCreg Name
SUSMi005-A-4
Alternative name(s)
SNCA3X 3KO C1, SNCA3X 3KO C2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 18th January 2022
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Provider

Generator Stanford University School of Medicine (SUSM)
Owner Stanford University School of Medicine (SUSM)
Derivation country United States

External Databases

BioSamples SAMEA12312968
CLO CLO_0103730
Cellosaurus CVCL_C0GZ, CVCL_C0GY
Wikidata Q112930441, Q112930442

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity White

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
obsolete_Parkinson's disease
The donor is a carrier of a disease-associated mutation and affected.
Stage
Hoehn & Yahr stage 3, moderate disease
Synonyms
  • Parkinson's syndrome
  • Parkinsons
  • Primary Parkinsonism
  • Parkinsons disease
  • Parkinson disease
  • Parkinson's disease (disorder)
  • Parkinson's disease NOS
  • Parkinson Disease, Idiopathic
  • PARKINSON DIS
  • IDIOPATHIC PARKINSONS DIS
  • PARKINSON DIS IDIOPATHIC
  • Parkinsonism, Primary
  • Parkinson's Disease, Lewy Body
  • IDIOPATHIC PARKINSON DIS
  • Idiopathic PD
  • Idiopathic Parkinson Disease
  • Lewy Body Parkinson's Disease
  • Parkinsonian disorder
  • LEWY BODY PARKINSON DIS
  • Parkinson's
  • Idiopathic Parkinson's Disease
  • Parkinson's disease NOS (disorder)
  • Lewy Body Parkinson Disease
  • PARKINSONS DIS IDIOPATHIC
  • PARKINSONS DIS
  • Parkinson's Disease, Idiopathic
  • Parkinson syndrome
  • PARKINSONS DIS LEWY BODY
  • Paralysis agitans
show more synonyms
Genetic variants
4q21
chr4, g.89337388-91047347trip (GRCh37/hg19)
Heterozygous
Size of mutation was detected by CGH and Bionano optical mapping
Family history positive
Is the medical history available upon request? Yes, see Byers et al. 2011
Is clinical information available? Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12279540

Ethics

Also have a look at the ethics information for the parental line SUSMi005-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line SUSMi005-A.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR
Notes on reprogramming vector silencing See Byers et al 2011

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex (ThermoFisher A3349401)
Main protein source: StemFlex supplement
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
HMP19
Unknown
FOXP2
Unknown
SST
Unknown
FOXA1
Unknown
AFP
Unknown
CDH20
Unknown
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
RGS4
Unknown
FOXF1
Unknown
CDX2
Unknown
ESM1
Unknown
HAND2
Unknown
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
LMX1A
Unknown
MAP2
Unknown
NES
Unknown
PAX6
Unknown
NR2F1
Unknown

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 59

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
obsolete_Parkinson's disease
Synonyms
  • Parkinson's syndrome
  • Parkinsons
  • Primary Parkinsonism
  • Parkinsons disease
  • Parkinson disease
  • Parkinson's disease (disorder)
  • Parkinson's disease NOS
  • Parkinson Disease, Idiopathic
  • PARKINSON DIS
  • IDIOPATHIC PARKINSONS DIS
  • PARKINSON DIS IDIOPATHIC
  • Parkinsonism, Primary
  • Parkinson's Disease, Lewy Body
  • IDIOPATHIC PARKINSON DIS
  • Idiopathic PD
  • Idiopathic Parkinson Disease
  • Lewy Body Parkinson's Disease
  • Parkinsonian disorder
  • LEWY BODY PARKINSON DIS
  • Parkinson's
  • Idiopathic Parkinson's Disease
  • Parkinson's disease NOS (disorder)
  • Lewy Body Parkinson Disease
  • PARKINSONS DIS IDIOPATHIC
  • PARKINSONS DIS
  • Parkinson's Disease, Idiopathic
  • Parkinson syndrome
  • PARKINSONS DIS LEWY BODY
  • Paralysis agitans
show more synonyms
Genetic modifications
SNCA (target)
Gene knock-out
4q21
CRISPR-associated (CRISPR/Cas) System