LCSBi012-A-1

RHOT1_T610A_clone62.19.37_IsogenicControl

General

Cell Line

hPSCreg name LCSBi012-A-1
Cite as:
LCSBi012-A-1 (RRID:CVCL_D0HK)
Alternative name(s)
RHOT1_T610A_clone62.19.37_IsogenicControl
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
LCSBi012-A
(RHOT1_T610A_clone6_PD)
Donor diseases:
Parkinson Disease
LCSBi011-A-1
(RHOT1_T351A_clone25.2_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi009-A-1
(RHOT1_R272Q_clone18_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi010-A-1
(RHOT1_R450C_clone6_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi008-A-1
(delP GC13, DJ-1-delP GC13)
Donor diseases:
Parkinson Disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-2
(SNCA3X 1KO C2, SNCA3X 1KO C1)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-4
(SNCA3X 3KO C1, SNCA3X 3KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-5
(SNCA3X 4KO C1, SNCA3X 4KO C2)
Donor diseases:
obsolete_Parkinson's disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-4
(AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
MPIi003-A-1
(IM2GC, L2-2GC)
Donor diseases:
obsolete_Parkinson's disease
EDi001-B-1
(AST18-7A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-2
(AST18-7B)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-3
(AST18-5D)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-4
(AST18-6A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-A-5
(AST23-2KO-II8B)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
LCSBi010-A-2
(RHOT1_R450C_clone10_IsogenicControl)
Donor diseases:
Parkinson Disease
ICGi015-B-1
(m6.7pCyto-17)
Donor diseases:
Parkinson Disease
ICGi015-B-2
(m6.7pCyto-21)
Donor diseases:
Parkinson Disease
ICGi015-B-3
(m6.7pCyto-24)
Donor diseases:
Parkinson Disease
ICGi034-A-1
(PD30-XBP-RFP-6, PD30-4-7-XBP-RFP-6)
Donor diseases:
obsolete_Parkinson's disease
ICGi034-A-2
(PD30-4-7-XBP-RFP-51, PD30-XBP-RFP-51)
Donor diseases:
obsolete_Parkinson's disease
STBCi090-B
(SFC867-04-12)
Donor diseases:
Parkinson disease
PNUSCRi001-A
(GBA PD iPSC7)
Donor's gene variants:
GBA
Donor diseases:
Parkinson Disease
LCSBi008-A
(delP, DJ-1-delP)
Donor diseases:
Parkinson Disease
PNUSCRi002-A
(GBA PD iPSC9)
Donor's gene variants:
GBA
Donor diseases:
Parkinson Disease
ICGi042-A
(PD12-4Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ICGi042-B
(PD12-5Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ICGi042-C
(PD12-6Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
STBCi005-B
(SFC833-03-05)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-C
(SFC833-03-14)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-B
(SFC855-03-08)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-C
(SFC855-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
LCSBi001-A
(VPS35 1_2)
Donor diseases:
obsolete_Parkinson's disease
LCSBi013-A
(GL2)
Donor diseases:
Parkinson Disease
CDIi013-A
(PPMI_3409, FCDI_11287)
Donor diseases:
Parkinson Disease
CDIi014-A
(FCDI_11292, PPMI_4055)
Donor diseases:
Parkinson Disease
CDIi017-A
(PPMI_3446, FCDI_11296)
Donor diseases:
Parkinson Disease
CDIi018-A
(PPMI_3234, FCDI_11298)
Donor diseases:
Parkinson Disease
CDIi019-A
(PPMI_52062, FCDI_11299)
Donor diseases:
Parkinson Disease
CDIi021-A
(PPMI_3459, FCDI_11301)
Donor diseases:
Parkinson Disease
CDIi023-A
(FCDI_11303, PPMI_3448)
Donor diseases:
Parkinson Disease
CDIi025-A
(FCDI_11306, PPMI_40273)
Donor diseases:
Parkinson Disease
CDIi029-A
(PPMI_3665, FCDI_11310)
Donor diseases:
Parkinson Disease
CDIi031-A
(PPMI_3451, FCDI_11312)
Donor diseases:
Parkinson Disease
Last update 21st March 2023
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Provider

Generator Luxembourg Centre for Systems Biomedicine (LCSB)

External Databases

BioSamples SAMEA112818019
Cellosaurus CVCL_D0HK
Wikidata Q123032941

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Align with cell line owner for Material Transfert agreement
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease

External Databases (Donor)

BioSamples SAMEA112817622

Ethics

Also have a look at the ethics information for the parental line LCSBi012-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line LCSBi012-A.

Reprogramming method

Vector type Integrating
Vector Plasmid
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Methods used
PCR
Notes on reprogramming vector silencing See parental line LCSBi012-A

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
Brachiury
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
OTX2
Yes

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
This line has been corrected, thus is used as an isogenic control of the Parkinson's disease patient-derived line LCSBi012-A
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
RHOT1 (target)
Isogenic modification
Homozygous
Correction of the point mutation via Crispr-Cas9
Repaired