hPSCreg®
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STBCi004-B-1

Registration Summary:

SFC832-03-06 LRRK2WT/WT C47

STBCi004-B-1

General

Cell Line
hPSCreg name STBCi004-B-1
Cite as:
STBCi004-B-1 (RRID:CVCL_A8X1)
Alternative name(s)
SFC832-03-06 LRRK2WT/WT C47
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi004-B
(SFC832-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-C
(SFC832-03-07)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-A
(SFC832-03-19)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
MPIi003-A-1
(IM2GC, L2-2GC)
Donor diseases:
obsolete_Parkinson's disease
SHEHDNi002-A
(KY03AP)
Donor's gene variants:
LRRK2, DNAJC6
Donor diseases:
Parkinson Disease
UOXFi007-A
(MK002-4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-B
(MK002-6)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-C
(MK002-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-A
(MK144-1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-B
(MK144-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-C
(MK144-10)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
DANi011-A
(LRRK2-011-C1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi042-A
(PD12-4Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ICGi042-B
(PD12-5Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ICGi042-C
(PD12-6Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ESi006-A
(SP13#4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-B
(SFC833-03-05)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-C
(SFC833-03-14)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-B
(SFC855-03-08)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-C
(SFC855-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
ICGi043-A
(LR-21)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi043-B
(LR-2)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi043-C
(LR-15)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi023-A
(PD45-6-1Lm)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
STBCi005-A
(SFC833-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-A
(SFC855-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
DANi005-A
(LRRK2-GBA-005-C1)
Donor's gene variants:
GBA, LRRK2
Donor diseases:
Parkinson Disease
STBCi026-A-1
(SFC840-03-03 LRRK2-/-D10)
STBCi026-A-2
(SFC840-03-03 LRRK2-/-C11)
STBCi026-A-3
(SFC840-03-03 LRRK2 WT/R1441C H3)
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-4
(AST22-2KO-6, AST23_SNCAKO Clone 6, AST22_SNCAKO Clone 6, AST23-2KO-6)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-B-1
(AST18-7A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-2
(AST18-7B)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-3
(AST18-5D)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-B-4
(AST18-6A)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-A-5
(AST23-2KO-II8B)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
LCSBi009-A-1
(RHOT1_R272Q_clone18_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi010-A-1
(RHOT1_R450C_clone6_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi010-A-2
(RHOT1_R450C_clone10_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi012-A-1
(RHOT1_T610A_clone62.19.37_IsogenicControl)
Donor diseases:
Parkinson Disease
LCSBi008-A-1
(delP GC13, DJ-1-delP GC13)
Donor diseases:
Parkinson Disease
SUSMi005-A-1
(SNCA3X 0KO C1, SNCA3X 0KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-2
(SNCA3X 1KO C2, SNCA3X 1KO C1)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-4
(SNCA3X 3KO C1, SNCA3X 3KO C2)
Donor diseases:
obsolete_Parkinson's disease
SUSMi005-A-5
(SNCA3X 4KO C1, SNCA3X 4KO C2)
Donor diseases:
obsolete_Parkinson's disease
ICGi015-B-1
(m6.7pCyto-17)
Donor diseases:
Parkinson Disease
Last update 8th December 2022
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Provider
Generator StemBANCC (STBC)
Distributors

External Databases
BioSamples SAMEA5859478
Cellosaurus CVCL_A8X1
Wikidata Q102114943

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Parkinson disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
LRRK2 (target)
12q12
NM_198578.3:c.6055G>A
NP_940980.3:p.Gly2019Ser
LRRK2 G2019S

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA104129730

Ethics

Also have a look at the ethics information for the parental line STBCi004-B .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line STBCi004-B.

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Spontaneous EB differentiation and qPCR for trilineage markers
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
VIM
Yes
DCN
Yes
MLX1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
HES5
Yes
NEUROD1
Yes
PAX6
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis
Is there a certificate of analysis available?
Yes
SFC832-03-06_LRRK2_WTWT_C47.pdf
Passage:

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
Karyotype abnormalities: none compared to fibroblasts
Passage number: 40
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson disease
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic modifications
LRRK2 (target)
Isogenic modification
12q12
The G2019S variant (NM_198578.3:c.6055G>A) in the gene LRRK2 was normalized by gene editing, resulting in the wt form: NM_198578.3:c.6055G. CRISPR/Cas9-mediated double-strand break generated close to G2019S mutation and homology-directed repair with donor sequence. Homology arms generated by amplification of LRRK2 sequence in mutant allele to maintain isogeneic sequence. The G2019S mutation was repaired (G>A), and other silent mutations introduced including mutated protospacer adjacent motifs to prevent recutting of repaired sequence, and a PstI site for identification of repaired clones. See Certificate of Analysis.
Repaired