SP13#4
ESi006-A
General#
Cell Line |
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hPSCreg Name | ESi006-A |
Alternative name(s) |
SP13#4
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Spanish Stem Cell Bank (ES) |
Distributors | |
Derivation country | Spain |
External Databases |
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BioSamples | SAMEA3303225 |
Cellosaurus | CVCL_V203 |
EBiSC | ESi006-A |
ECACC | 66540068 |
General Information |
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Publications | |
Projects | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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External Databases (Donor) |
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BioSamples | SAMEA3303187 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
keratinocyteAn epidermal cell which synthesizes keratin and undergoes a characteristic change as it moves upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Synonyms
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Age of donor (at collection) | 65-69 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
Unkown |
Absence of reprogramming vector(s)? |
Unkown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Gelatin | |||||||||||||||
Feeder cells |
Human foreskin fibroblasts Cellfinder Ont Id: CELDA_000001419 |
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Passage method | Mechanically | |||||||||||||||
CO2 Concentration | 5 % | |||||||||||||||
Medium |
Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-3 |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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SOX2 |
Yes |
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Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
SMA |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
Marker | Expressed |
Tuj1 |
Yes |
GFAP |
Yes |
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
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