SFC831-03-01

General

Cell Line

hPSCreg name STBCi024-A
Cite as:
STBCi024-A (RRID:CVCL_RB79)
Alternative name(s)
SFC831-03-01
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi024-C
(SFC831-03-05)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi024-B
(SFC831-03-03)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
STBCi019-C
(SFC828-03-04)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi019-A
(SFC828-03-06)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi019-B
(SFC828-03-09)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi023-B
(SFC829-03-04)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi083-B
(SFC830-04-08)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi008-B
(G51D-4, EDINi008-B, EDIi008-B, SAMEA3174606)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
STBCi023-A
(SFC829-03-02)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi023-C
(SFC829-03-06)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi083-A
(SFC830-04-09)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
EDi001-A
(AST22, AST23, SAMEA3319992)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi084-A
(SFC871-03-12)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi084-C
(SFC871-03-09)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi004-A
(SFC832-03-19)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi084-B
(SFC871-03-04)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi004-B
(SFC832-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-C
(SFC832-03-07)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi266-A
(SFC072-03-06)
Donor diseases:
Parkinson disease
STBCi278-A
(SFC245-03-08)
Donor diseases:
Parkinson disease
STBCi280-A
(SFC066-03-01)
Donor diseases:
Parkinson disease
STBCi283-A
(SFC073-03-08)
Donor diseases:
Parkinson disease
STBCi292-A
(SFC139-03-06)
Donor diseases:
Parkinson disease
STBCi293-A
(SFC843-03-09)
Donor diseases:
Parkinson disease
STBCi297-A
(SFC061-03-07)
Donor diseases:
Parkinson disease
STBCi025-C
(SFC834-03-10)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi085-A
(SFC866-03-06)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi088-A
(SFC872-03-05)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi267-A
(SFC027-03-02)
Donor diseases:
Parkinson disease
STBCi005-A
(SFC833-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-A
(SFC855-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi002-B
(MK082-30)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi025-B
(SFC834-03-03)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi040-A
(SFC029-03-04)
Donor diseases:
Parkinson disease
STBCi040-B
(SFC029-03-08)
Donor diseases:
Parkinson disease
STBCi041-A
(SFC081-03-02)
Donor diseases:
Parkinson disease
STBCi042-A
(SFC848-03-02)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi042-B
(SFC848-03-04)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi042-C
(SFC848-03-06)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi043-A
(SFC120-03-03)
Donor diseases:
Parkinson disease
STBCi043-B
(SFC120-03-04)
Donor diseases:
Parkinson disease
STBCi040-C
(SFC029-03-01)
Donor diseases:
Parkinson disease
STBCi041-B
(SFC081-03-04)
Donor diseases:
Parkinson disease
STBCi043-C
(SFC120-03-02)
Donor diseases:
Parkinson disease
UOXFi001-B
(MK071-3)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi320-A
(SFC031-03-03)
Donor diseases:
Parkinson disease
Last update 9th January 2024
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Provider

Generator StemBANCC (STBC)
Distributors

External Databases

BioSamples SAMEA104493652
Cellosaurus CVCL_RB79
Wikidata Q54956400

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
SNCA (target)
4q22.1
SNCA triplication

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493653

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information zameel.cader@ndcn.ox.ac.uk
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Royal Free Hospital and Medical School Research Ethics Committee
Approval number 07/H0720/161
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Royal Free Hospital and Medical School Research Ethics Committee
Approval number 07/H0720/161
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: Reseach use restriction , only to be used in Parkinson's Disease. Material shall not be sold, transplanted into any human being or used to create egg or sperm cells (gametes) or embryos. The material shall not be used for direct exploitation. For the purposes of this, Direct exploitation means to develop for commericalization or to commercialize the Material.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 55-59

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 25

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)