The cell line is not validated yet.
LCSBi001-A-2
General
Cell Line |
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| hPSCreg name | LCSBi001-A-2 |
| Cite as: | LCSBi001-A-2 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 30th January 2026 |
| User feedback | |
Provider |
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| Generator | Luxembourg Centre for Systems Biomedicine (LCSB) |
| Owner | Luxembourg Centre for Systems Biomedicine (LCSB) |
| Distributors | |
| Derivation country | Luxembourg |
External Databases |
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| BioSamples | SAMEA120246468 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
A Material Transfer Agreement between the provider and the recipient will be necessary for the sharing of the cell line. |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA6673734 |
Ethics
Also have a look at the ethics information for the parental line
LCSBi001-A
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
LCSBi001-A.
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| Passage number reprogrammed | p6 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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Vector free reprogramming |
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Other |
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| Selection criteria for clones | morphology |
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| CO2 Concentration | 5 % | ||||||
| Medium |
mTeSR™ Plus
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| NANOG |
Yes |
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| DNMT3B |
Yes |
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Morphology pictures
Compact colony with sharp edges (scale bar: 200 um)
Differentiation Potency
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XY
Karyotyping method:
Array CGH
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Other Genotyping (Cell Line) |
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Genetic Modification
| Disease/phenotype related modifications |
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