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ICGi043-A-1

Registration Summary:

LR-21-cytoGRX-3c

ICGi043-A-1

General

Cell Line
hPSCreg name ICGi043-A-1
Cite as:
ICGi043-A-1
Alternative name(s)
LR-21-cytoGRX-3c
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
ICGi043-A
(LR-21)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi043-B
(LR-2)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi043-C
(LR-15)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
CBIGi013-A-1
(LRRK2 M1646T correction, IPSC0018)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi027-A-1
(IPSC0039, LRRK2 G2385R (CBIGi027) correction)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
CBIGi039-A-1
(IPSC0051, LRRK2 R1441H(CBIGi039-A) correction)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi044-A-1
(IPSC0059, LRRK2 N551K (CBIGi044-A) correction)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi044-A-2
(IPSC0060, LRRK2 R1398H (CBIGi044-A) correction)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
ICGi015-B-1
(m6.7pCyto-17)
Donor diseases:
Parkinson Disease
ICGi015-B-2
(m6.7pCyto-21)
Donor diseases:
Parkinson Disease
ICGi015-B-3
(m6.7pCyto-24)
Donor diseases:
Parkinson Disease
ICGi034-A-1
(PD30-XBP-RFP-6, PD30-4-7-XBP-RFP-6)
Donor diseases:
obsolete_Parkinson's disease
ICGi034-A-2
(PD30-4-7-XBP-RFP-51, PD30-XBP-RFP-51)
Donor diseases:
obsolete_Parkinson's disease
SHEHDNi002-A
(KY03AP)
Donor's gene variants:
LRRK2, DNAJC6
Donor diseases:
Parkinson Disease
CBIGi005-A
(IPSC0003, LRRK2 G2019S)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi013-A
(IPSC0017, LRRK2 M1646T (heterozygous))
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi015-A
(IPSC0020, LRRK2 N551K-R1398H-K1423K (protective haplotype))
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi027-A
(LRRK2 G2385R (CBIGi027), IPSC0037)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
STBCi004-B
(SFC832-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-C
(SFC832-03-07)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-A
(MK002-4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-B
(MK002-6)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi007-C
(MK002-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-A
(MK144-1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-B
(MK144-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-C
(MK144-10)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi004-A
(SFC832-03-19)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
DANi011-A
(LRRK2-011-C1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ESi006-A
(SP13#4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
ICGi023-A
(PD45-6-1Lm)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson Disease
ICGi042-A
(PD12-4Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ICGi042-B
(PD12-5Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
ICGi042-C
(PD12-6Lm)
Donor's gene variants:
LRRK2, PINK1
Donor diseases:
Parkinson Disease
STBCi005-B
(SFC833-03-05)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-C
(SFC833-03-14)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-B
(SFC855-03-08)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-C
(SFC855-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
CBIGi037-A
(IPSC0048, LRRK2 M1646T(CBIGi037-A))
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi039-A
(IPSC0050, LRRK2 R1441H(CBIGi039-A))
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi040-A
(IPSC0052, LRRK2 N551K-R1398H-K1423K (protective haplotype) (CBIGi040-A))
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
CBIGi044-A
(LRRK2 N551K-R1398H-K1423K (protective haplotype)(CBIGi044-A), IPSC0058)
Donor's gene variants:
LRRK2, LRRK2
Donor diseases:
Parkinson Disease
STBCi005-A
(SFC833-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-A
(SFC855-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
DANi005-A
(LRRK2-GBA-005-C1)
Donor's gene variants:
GBA, LRRK2
Donor diseases:
Parkinson Disease
ICGi034-A-3
(PD30-4-7-XBP-RFP-52, PD30-XBP-RFP-52)
Donor diseases:
obsolete_Parkinson's disease
ICGi034-A-4
(PD30-4-7-XBP-RFP-86, PD30-XBP-RFP-86)
Donor diseases:
obsolete_Parkinson's disease
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
Last update 4th July 2024
Notes iPSC clone of an LRRK2-assotiated Parkinson's disease patient with doxycycline-inducible expression of cytosolic Grx1-roGFP2
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Provider
Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases
BioSamples SAMEA115802956

General Information
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: Any
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information
Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Parkinson Disease
Clinical diagnosis: LRRK2-associated Parkinson's disease, tremulous form, stage 2.0 on the Hoehn and Yahr scale, with predominant involvement of the right limbs, complicated by motor fluctuations (single dose depletion phenomenon, morning akinesia), mild postural deformity with developing camptocormia, mild mental disorders. Disease duration: 5 years
The donor is a carrier of a disease-associated mutation and affected.
Stage
Stage 2.0 on the Hoehn and Yahr scale
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
LRRK2 (target)
12q12
NM_198578.4(LRRK2):c.6055G>A
NP_940980.4:p.Gly2019Ser
Heterozygous
VCV000001940.40
rs34637584
Family history Father's cousin had a tremor

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA112177268

Ethics

Also have a look at the ethics information for the parental line ICGi043-A .
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
The source cell information can be found in the parental cell line ICGi043-A.

Reprogramming method
Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Gelatin
Feeder cells MEF
Cellfinder Ont Id: http://www.ebi.ac.uk/efo/EFO_0004040
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knock-out DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK-18
Yes
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
CD29
Yes
aSMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Neurofilament 200
Yes
MAP2
Yes

Microbiology / Virus Screening
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Parkinson Disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic modifications
Genetic modifications not related to a disease
AAVS1 (target)
Transgene expression
19q13
Transgenes of glutathione redox sensor (cytosolic) cyto_Grx1-roGFP2 and tetracycline-transactivator (rtTA) in AAVS1 locus were detected by PCR.
CRISPR-associated (CRISPR/Cas) System