PD12-4Lm

General

Cell Line

hPSCreg Name
ICGi042-A
Alternative name(s)
PD12-4Lm
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 24th August 2022
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Provider

Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA110644571
CLO CLO_0103970
Cellosaurus CVCL_C1TN

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 75-79
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's Disease
The donor is a carrier of a disease-associated mutation and affected.
Stage
3-4 stage of Parkinson's disease
Synonyms
  • Parkinson Disease
  • Parkinson's disease
  • Parkinson's Disease
Genetic variants
LRRK2 (target)
12q12
Heterozygous
VCV000039128.22, VCV000039146.8
PD12 L3 LRRK2.tif
LRRK2, rs78501232
PD12 L7 LRRK2.tif
LRRK2, rs10878307
PINK1 (target)
1p36.12
Heterozygous
VCV000295006.25
PD12 P2 PINK1.tif
PINK1, rs1043424

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMN14446263

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? FSBI Federal Neurosurgical Center
Approval number Protocol number 1 14/03/2017
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Source cell origin
Peripheral Blood
Blood drawn from a limb.
Synonyms
  • Peripheral Blood
Age of donor (at collection) 75-79
Collected in 2015
Source cell line vendor FSBI Federal Neurosurgical Center
Passage number reprogrammed 1

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones The selection of colonies was carried out according to morphological criteria. We selected flat monolayer colonies with tightly packed cells with high nuclear/cytoplasmic ratio. Embryonic stem cell-like clones were picked by a glass microcapillary.
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
TRA 1-60
Yes
Morphology pictures
Morphol PD12-4m, 12p.tif
Morphology of ICGi042-A iPSC at passage 12
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CK18
Yes
GATA6
Yes
Morphology
Sp dif PD12-4Lm, 15p CK18_r GATA6_g.tif
Immunofluorescent analysis for endoderm markers GATA6 (green signal) and CYTOKERATIN 18 (red signal) in ICGi042-A iPSC at passage 15. DAPI (blue signal)
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Morphology
Sp dif PD12-4Lm, 15p aSMA_r Coll4_g.tif
Immunofluorescent analysis for mesoderm markers alfa SMA (red signal) and COLLAGEN 4 (green signal) in ICGi042-A iPSC at passage 15. DAPI (blue signal)
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes
Neurofilament 200
Yes
Morphology
Sp dif PD12-4Lm, 15p bIII_r NF200_g.tif
Immunofluorescent analysis for ectoderm marker TUBB3 (red signel) and NEUROFILAMENT 200 in ICGi042-A iPSC at passage 15. DAPI (blue signal)

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotyping and G-banding show ICGi042-A iPSCs have a normal 46, XY karyotype.
Passage number: 16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)